Loading…
Unraveling the dha cluster in Citrobacter werkmanii: comparative genomic analysis of bacterial 1,3-propanediol biosynthesis clusters
In natural 1,3-propanediol (PDO) producing microorganisms such as Klebsiella pneumoniae , Citrobacter freundii and Clostridium sp., the genes coding for PDO producing enzymes are grouped in a dha cluster. This article describes the dha cluster of a novel candidate for PDO production, Citrobacter wer...
Saved in:
Published in: | Bioprocess and biosystems engineering 2014-04, Vol.37 (4), p.711-718 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | In natural 1,3-propanediol (PDO) producing microorganisms such as
Klebsiella pneumoniae
,
Citrobacter freundii
and
Clostridium
sp., the genes coding for PDO producing enzymes are grouped in a
dha
cluster. This article describes the
dha
cluster of a novel candidate for PDO production,
Citrobacter werkmanii
DSM17579 and compares the cluster to the currently known PDO clusters of
Enterobacteriaceae
and
Clostridiaceae
. Moreover, we attribute a putative function to two previously unannotated ORFs, OrfW and OrfY, both in
C. freundii
and in
C. werkmanii
: both proteins might form a complex and support the glycerol dehydratase by converting cob(I)alamin to the glycerol dehydratase cofactor coenzyme B
12
. Unraveling this biosynthesis cluster revealed high homology between the deduced amino acid sequence of the open reading frames of
C. werkmanii
DSM17579 and those of
C. freundii
DSM30040 and
K. pneumoniae
MGH78578, i.e., 96 and 87.5Â % identity, respectively. On the other hand, major differences between the clusters have also been discovered. For example, only one dihydroxyacetone kinase (DHAK) is present in the
dha
cluster of
C. werkmanii
DSM17579, while two DHAK enzymes are present in the cluster of
K. pneumoniae
MGH78578 and
Clostridium butyricum
VPI1718. |
---|---|
ISSN: | 1615-7591 1615-7605 |
DOI: | 10.1007/s00449-013-1041-0 |