Differential expression analysis of Golgi apparatus proteomes in hepatocellular carcinomas and the surrounding liver tissues

Aim Hepatocellular carcinoma (HCC) is the sixth most common malignancy worldwide. Liver is the largest human digestive gland with abundant Golgi apparatus involved in cell division, migration and apoptosis and others. Methods In the present study, Golgi apparatus of HCC and the surrounding liver tis...

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Bibliographic Details
Published in:Hepatology research 2014-05, Vol.44 (5), p.542-550
Main Authors: Yang, Yaying, Luo, Yi, Li, Xiujun, Yi, Yongfen
Format: Article
Language:English
Subjects:
2-D electrophoresis
Golgi apparatus
hepatocellular carcinoma
mass spectrometry
proteomes
Online Access:Get full text
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Summary:Aim Hepatocellular carcinoma (HCC) is the sixth most common malignancy worldwide. Liver is the largest human digestive gland with abundant Golgi apparatus involved in cell division, migration and apoptosis and others. Methods In the present study, Golgi apparatus of HCC and the surrounding liver tissues were isolated by sucrose density gradient centrifugation and identified by electron microscopy and enzymology methods. Using 2‐D gel electrophoresis and mass spectrometry, 17 differentially expressed protein of Golgi apparatus in HCC and the surrounding liver tissue were screened and identified in the Mascot database. Results Of those differentially expressed proteins, six were upregulated and 11 were downregulated, some of them were related to the biological processes such as protein sorting, glycosylation, cell cycle regulation, transcription regulation and Golgi integrity. One protein, annexin A5, was verified to be upregulated in HCC by western blot. Conclusion The differentially expressed proteins may provide new insight into HCC biology and potential diagnostic and therapeutic biomarkers.
ISSN:1386-6346
1872-034X
DOI:10.1111/hepr.12151