An exonuclease possibly involved in the initiation of repair of bleomycin-damaged DNA in mouse ascites sarcoma cells

A protein factor having exonucleolytic activity on bleomycindamaged DNA and providing priming sites for DNA polymerasesexisted in a DNA polymerase β fraction partially purified by ion exchange chromatography from an extract of permeable mouse ascites sarcoma (SR-C3H/He) cells. The exonuclease was se...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1988-12, Vol.9 (12), p.2239-2244
Main Authors: Seki, Shuji, Oda, Takuzo
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Bleomycin - pharmacology
DNA - biosynthesis
DNA - drug effects
DNA Damage
DNA Polymerase I - metabolism
DNA Repair
Exonucleases - physiology
General aspects
Iron - pharmacology
Medical sciences
Mice
Pharmacology. Drug treatments
Sarcoma, Experimental - metabolism
Templates, Genetic
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Summary:A protein factor having exonucleolytic activity on bleomycindamaged DNA and providing priming sites for DNA polymerasesexisted in a DNA polymerase β fraction partially purified by ion exchange chromatography from an extract of permeable mouse ascites sarcoma (SR-C3H/He) cells. The exonuclease was separated from DNA polymerase β by singlestranded DNA-cellulose chromatography, and partially characterized. The enzyme is suggestedto be involved in the initial step of repair of bleomycin-damaged DNA in removing 3′ ends (3′-phosphoglycolate termini) of bleomycin-damaged DNA.
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/9.12.2239