Potential antidiabetic activity of extracellular polysaccharides in submerged fermentation culture of Coriolus versicolor LH1

• Published in: Carbohydrate polymers 2012-09, Vol.90 (1), p.174-180
• Main Authors: Yang, John Powen, Hsu, Taihao, Lin, Fangyi, Hsu, Wenkuang, Chen, Yucheng
• Format: Article
• Language: English
• Subjects: alpha-glucosidase; anion exchange; Applied sciences; Biological and medical sciences; Coriolaceae; Coriolus versicolor; Coriolus versicolor LH1; culture media; enzyme inhibition; Exact sciences and technology; Extracellular polysaccharides; Fermentation - physiology; gel chromatography; General and cellular metabolism. Vitamins; glycemic effect; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - metabolism; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polyporaceae; polysaccharides; Polysaccharides - isolation & purification; Polysaccharides - metabolism; proteins; Starch and polysaccharides; submerged fermentation; Trametes - metabolism; Triterpenoides; α-Glucosidase inhibition
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.05.011

► LH1 is a new strain of Coriolus versicolor. ► We have separated and purified three polysaccharides from C. versicolor LH1. ► These polysaccharides could be explored as a novel potential antidiabetic. ► These polysaccharides included carbohydrates, triterpenoids and/or proteins. ► The ratio of polysaccharides–triterpenoids is related to α-glucosidase inhibition. The separation and purification of extracellular polysaccharides from Coriolus versicolor LH1 were investigated along with their α-glucosidase inhibition properties. Three polysaccharide fractions (ePS-F2-1, ePS-F3-1, and ePS-F4-1) were separated from the culture medium of LH1 using a DEAE anion-exchange column and a Sephadex™ G-50 gel filtration column. Their chemical compositions was determined. On the basis of an α-glucosidase inhibition assay, the enzyme inhibition activities of ePS-F2-1, ePS-F3-1, and ePS-F4-1 were investigated. Among these, ePS-F4-1 had the highest enzyme inhibition effects on α-glucosidase. According to the results of the chemical component analysis, ePS-F3-1 and ePS-F4-1 are the polysaccharides which are combined with triterpenoides, and ePS-F2-1 is complexed with proteins and triterpenoides.