Cytochrome b sub(2), an electron carrier between flavocytochrome b sub(2) and cytochrome c: Rapid kinetic characterization of the electron-transfer parameters with ionic-strength-dependence

The oxidation-reduction properties of free cytochrome b sub(2) Isolated by controlled proteolysis from flavocytochrome b sub(2), i.e. the flavodehydrogenase-bound cytochrome b sub(2) were investigated by using stopped-flow spectrophotometry. The rapid kinetics of the reduction of cytochrome b sub(2)...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical journal 1987-01, Vol.245 (1), p.159-165
Main Authors: Capeillere-Blandin, C, Albani, J
Format: Article
Language:English
Subjects:
cytochrome b2
electron transfer
Hansenula anomala
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The oxidation-reduction properties of free cytochrome b sub(2) Isolated by controlled proteolysis from flavocytochrome b sub(2), i.e. the flavodehydrogenase-bound cytochrome b sub(2) were investigated by using stopped-flow spectrophotometry. The rapid kinetics of the reduction of cytochrome b sub(2) by flavocytochrome b sub(2) in presence of L-lactate are reported. The low reaction rate observed with fere cytochrome b sub(2) is ascribed to the low stability of the reaction complex formed between free cytochrome b sub(2) and cytochrome c.
ISSN:0264-6021