Electrostatic map of T7 DNA: comparative analysis of functional and electrostatic properties of T7 RNA polymerase-specific promoters

The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBI RefSeq N1001604). Here, electrostatic potential distribution around double helical T7 DNA was calculated by Coulomb method using the computer program of Sorokin A.A. (lptolik@gmail.com). Electrostatic profiles of 17 promoter...

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Bibliographic Details
Published in:Journal of biomolecular structure & dynamics 2014-08, Vol.32 (8), p.1184-1192
Main Authors: Kamzolova, S.G., Beskaravainy, P.M., Osypov, A.A., Dzhelyadin, T.R., Temlyakova, E.A., Sorokin, A.A.
Format: Article
Language:English
Subjects:
Bacteriophage T7 - enzymology
Bacteriophage T7 - genetics
DNA electrostatics
DNA, Viral - chemistry
DNA-Directed RNA Polymerases - genetics
Genome, Viral
phage T7
promoter
Promoter Regions, Genetic
Static Electricity
Viral Proteins - genetics
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Summary:The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBI RefSeq N1001604). Here, electrostatic potential distribution around double helical T7 DNA was calculated by Coulomb method using the computer program of Sorokin A.A. (lptolik@gmail.com). Electrostatic profiles of 17 promoters recognized by T7 phage-specific RNA polymerase were analyzed. It was shown that electrostatic profiles of all T7 RNA polymerase-specific promoters can be characterized by distinctive motifs which are specific for each promoter class. Comparative analysis of electrostatic profiles of native T7 promoters of different classes demonstrates that T7 RNA polymerase can differentiate them due to their electrostatic features.
ISSN:0739-1102
1538-0254
DOI:10.1080/07391102.2013.819298