Evaluation of silica cartridge purification and hemiacetal formation for liquid chromatographic determination of aflatoxins in corn

Rapid silica cartridge cleanup, acid-catalyzed conversion of aflatoxins B1 and G1 to hemiacetals, and reverse-phase liquid chromatography with fluorescence detection were evaluated for effectiveness in determining aflatoxins B1, B2, G1, and G2 in corn at concentrations ranging from 2 ng/g to 100 mic...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 1989-05, Vol.18 (3), p.319-326
Main Authors: Hutchins, J.E, Lee, Y.J, Tyczkowska, K, Hagler, W.M. Jr
Format: Article
Language:English
Subjects:
AFLATOXINAS
AFLATOXINE
AFLATOXINS
Aflatoxins - analysis
ALIMENT POUR ANIMAUX
Biological and medical sciences
Cereal and baking product industries
Chemical Phenomena
Chemistry
CHROMATOGRAPHIE
CHROMATOGRAPHY
Chromatography, High Pressure Liquid - instrumentation
CROMATOGRAFIA
EPURATION
FEEDS
Food Contamination - analysis
FOOD HYGIENE
Food industries
Fundamental and applied biological sciences. Psychology
HIGIENE DE LOS ALIMENTOS
HYGIENE DES ALIMENTS
PIENSOS
PURIFICACION
PURIFICATION
SILICIUM
SILICON
ZEA MAYS
Zea mays - analysis
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Summary:Rapid silica cartridge cleanup, acid-catalyzed conversion of aflatoxins B1 and G1 to hemiacetals, and reverse-phase liquid chromatography with fluorescence detection were evaluated for effectiveness in determining aflatoxins B1, B2, G1, and G2 in corn at concentrations ranging from 2 ng/g to 100 micrograms/g. In testing the method, aflatoxins applied to silica cartridges were recovered at greater than 97.1% overall. Conversion of aflatoxins B1 and G1 to their hemiacetals was shown to be complete (150 micrograms of toxin per extract). Application of these techniques to spiked corn extracts yielded data indicating excellent repeatability of derivatization relative to paired standards; coefficients of variation ranged from 1.08% to 5.81%. The repeatability of the method with naturally contaminated corn was also excellent; coefficients of variation ranged from 1.15% to 3.97%. Liquid chromatographic determination of aflatoxins in corn using fluorescence detection was sensitive, accurate, and precise resulting in applicability from less than 1 ng/g of aflatoxin B1 to greater than 100,000 ng/g.
ISSN:0090-4341
1432-0703
DOI:10.1007/BF01062355