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Evaluation of silica cartridge purification and hemiacetal formation for liquid chromatographic determination of aflatoxins in corn

Rapid silica cartridge cleanup, acid-catalyzed conversion of aflatoxins B1 and G1 to hemiacetals, and reverse-phase liquid chromatography with fluorescence detection were evaluated for effectiveness in determining aflatoxins B1, B2, G1, and G2 in corn at concentrations ranging from 2 ng/g to 100 mic...

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Published in:	Archives of environmental contamination and toxicology 1989-05, Vol.18 (3), p.319-326
Main Authors:	Hutchins, J.E, Lee, Y.J, Tyczkowska, K, Hagler, W.M. Jr
Format:	Article
Language:	English
Subjects:	AFLATOXINAS AFLATOXINE AFLATOXINS Aflatoxins - analysis ALIMENT POUR ANIMAUX Biological and medical sciences Cereal and baking product industries Chemical Phenomena Chemistry CHROMATOGRAPHIE CHROMATOGRAPHY Chromatography, High Pressure Liquid - instrumentation CROMATOGRAFIA EPURATION FEEDS Food Contamination - analysis FOOD HYGIENE Food industries Fundamental and applied biological sciences. Psychology HIGIENE DE LOS ALIMENTOS HYGIENE DES ALIMENTS PIENSOS PURIFICACION PURIFICATION SILICIUM SILICON ZEA MAYS Zea mays - analysis
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description	Rapid silica cartridge cleanup, acid-catalyzed conversion of aflatoxins B1 and G1 to hemiacetals, and reverse-phase liquid chromatography with fluorescence detection were evaluated for effectiveness in determining aflatoxins B1, B2, G1, and G2 in corn at concentrations ranging from 2 ng/g to 100 micrograms/g. In testing the method, aflatoxins applied to silica cartridges were recovered at greater than 97.1% overall. Conversion of aflatoxins B1 and G1 to their hemiacetals was shown to be complete (150 micrograms of toxin per extract). Application of these techniques to spiked corn extracts yielded data indicating excellent repeatability of derivatization relative to paired standards; coefficients of variation ranged from 1.08% to 5.81%. The repeatability of the method with naturally contaminated corn was also excellent; coefficients of variation ranged from 1.15% to 3.97%. Liquid chromatographic determination of aflatoxins in corn using fluorescence detection was sensitive, accurate, and precise resulting in applicability from less than 1 ng/g of aflatoxin B1 to greater than 100,000 ng/g.
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The repeatability of the method with naturally contaminated corn was also excellent; coefficients of variation ranged from 1.15% to 3.97%. 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Psychology ; HIGIENE DE LOS ALIMENTOS ; HYGIENE DES ALIMENTS ; PIENSOS ; PURIFICACION ; PURIFICATION ; SILICIUM ; SILICON ; ZEA MAYS ; Zea mays - analysis</subject><ispartof>Archives of environmental contamination and toxicology, 1989-05, Vol.18 (3), p.319-326</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-50dfca8081a65da70b04bca67b67d1425023e7e77e59a35c56c0059d800259c63</citedby><cites>FETCH-LOGICAL-c362t-50dfca8081a65da70b04bca67b67d1425023e7e77e59a35c56c0059d800259c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19313635$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2730151$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hutchins, J.E</creatorcontrib><creatorcontrib>Lee, Y.J</creatorcontrib><creatorcontrib>Tyczkowska, K</creatorcontrib><creatorcontrib>Hagler, W.M. Jr</creatorcontrib><title>Evaluation of silica cartridge purification and hemiacetal formation for liquid chromatographic determination of aflatoxins in corn</title><title>Archives of environmental contamination and toxicology</title><addtitle>Arch Environ Contam Toxicol</addtitle><description>Rapid silica cartridge cleanup, acid-catalyzed conversion of aflatoxins B1 and G1 to hemiacetals, and reverse-phase liquid chromatography with fluorescence detection were evaluated for effectiveness in determining aflatoxins B1, B2, G1, and G2 in corn at concentrations ranging from 2 ng/g to 100 micrograms/g. In testing the method, aflatoxins applied to silica cartridges were recovered at greater than 97.1% overall. Conversion of aflatoxins B1 and G1 to their hemiacetals was shown to be complete (150 micrograms of toxin per extract). Application of these techniques to spiked corn extracts yielded data indicating excellent repeatability of derivatization relative to paired standards; coefficients of variation ranged from 1.08% to 5.81%. The repeatability of the method with naturally contaminated corn was also excellent; coefficients of variation ranged from 1.15% to 3.97%. 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Psychology</subject><subject>HIGIENE DE LOS ALIMENTOS</subject><subject>HYGIENE DES ALIMENTS</subject><subject>PIENSOS</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>SILICIUM</subject><subject>SILICON</subject><subject>ZEA MAYS</subject><subject>Zea mays - analysis</subject><issn>0090-4341</issn><issn>1432-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNpFkE1P3DAQhi3Uii4fF46VKvkCh0qBsR3HybFF0FZC4gCco1l_7Bo58WInqD3zxzHdFZxmNM-jd6SXkBMG5wxAXfy8BgYNF1LukQWrBa9AgfhEFgAdVLWo2RdykPMjAONtW--Tfa4EMMkW5OXqGcOMk48jjY5mH7xGqjFNyZuVpZs5eVdO_wUcDV3bwaO2EwbqYhq2oGw0-KfZG6rXKZZrXCXcrL2mxk42DX58f4EuFPzXj5n6keqYxiPy2WHI9ng3D8nD9dX95e_q5vbXn8sfN5UWDZ8qCcZpbKFl2EiDCpZQLzU2atkow2ougQurrFJWdiiklo0GkJ1pAbjsdCMOydk2d5Pi02zz1A8-axsCjjbOuS-FdCClKOL3rahTzDlZ12-SHzD96xn0b433H40X-dsudV4O1ryru4oLP91xzBqDSzhqnz8SO8FEI95yvm49h7HHVSrOw13bMSl4LV4BDYWSJQ</recordid><startdate>19890501</startdate><enddate>19890501</enddate><creator>Hutchins, J.E</creator><creator>Lee, Y.J</creator><creator>Tyczkowska, K</creator><creator>Hagler, W.M. 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subjects	AFLATOXINAS AFLATOXINE AFLATOXINS Aflatoxins - analysis ALIMENT POUR ANIMAUX Biological and medical sciences Cereal and baking product industries Chemical Phenomena Chemistry CHROMATOGRAPHIE CHROMATOGRAPHY Chromatography, High Pressure Liquid - instrumentation CROMATOGRAFIA EPURATION FEEDS Food Contamination - analysis FOOD HYGIENE Food industries Fundamental and applied biological sciences. Psychology HIGIENE DE LOS ALIMENTOS HYGIENE DES ALIMENTS PIENSOS PURIFICACION PURIFICATION SILICIUM SILICON ZEA MAYS Zea mays - analysis
title	Evaluation of silica cartridge purification and hemiacetal formation for liquid chromatographic determination of aflatoxins in corn
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