Patterned Substrates of Nano-Graphene Oxide Mediating Highly Localized and Efficient Gene Delivery

A facile approach was developed to fabricate patterned substrates of nano-graphene oxide, demonstrating highly localized and efficient gene delivery to multiple cell lines in a substrate-mediated manner. The GO substrates served as a valid platform to preconcentrate PEI/pDNA complexes and maintain t...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2014-04, Vol.6 (8), p.5900-5907
Main Authors: Li, Kunyang, Feng, Liangzhu, Shen, Jianwei, Zhang, Qi, Liu, Zhuang, Lee, Shuit-Tong, Liu, Jian
Format: Article
Language:English
Subjects:
Cell Line
Gene Transfer Techniques - instrumentation
Graphite - chemistry
Humans
Oxides - chemistry
Plasmids - chemistry
Plasmids - genetics
Plasmids - metabolism
Stem Cells - metabolism
Transfection
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Summary:A facile approach was developed to fabricate patterned substrates of nano-graphene oxide, demonstrating highly localized and efficient gene delivery to multiple cell lines in a substrate-mediated manner. The GO substrates served as a valid platform to preconcentrate PEI/pDNA complexes and maintain their gradual releasing for a relatively long period of time. Our approach allowed successful gene delivery in selected groups of cells on the stripe-patterned GO substrates, without transfecting their neighbor cells directly cultured on glass. These GO substrates exhibited excellent biocompatibility and enabled effective gene transfection for various cell lines including stem cells, thus promising important applications in stem cell research and tissue engineering.
ISSN:1944-8244
1944-8252
DOI:10.1021/am5008134