Plasma Activity of Endothelial Lipase Impacts High-Density Lipoprotein Metabolism and Coronary Risk Factors in Humans

Aim: Endothelial lipase(EL) is a determinant of plasma levels of high-density lipoprotein cholesterol(HDL-C). However, little is known about the impact of EL activity on plasma lipid profile. We aimed to establish a new method to evaluate EL-specific phospholipase activity in humans. Methods: Plasma...

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Published in:Journal of Atherosclerosis and Thrombosis 2014/04/24, Vol.21(4), pp.313-321
Main Authors: Sun, Li, Ishida, Tatsuro, Miyashita, Kazuya, Kinoshita, Noriaki, Mori, Kenta, Yasuda, Tomoyuki, Toh, Ryuji, Nakajima, Katsuyuki, Imamura, Shigeyuki, Hirata, Ken-ichi
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Animals
Cercopithecus aethiops
Cholesterol
Coronary artery disease
Coronary Artery Disease - metabolism
COS Cells
Endothelial lipase
Female
Fluorescent Dyes - chemistry
High-density lipoprotein
Humans
Immunoglobulin G - blood
Immunoprecipitation
Lipase - blood
Lipoproteins, HDL - blood
Male
Middle Aged
Myocardium - enzymology
Phospholipase
Phospholipases - metabolism
Phospholipids - metabolism
Recombinant Proteins - metabolism
Risk Factors
Young Adult
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Summary:Aim: Endothelial lipase(EL) is a determinant of plasma levels of high-density lipoprotein cholesterol(HDL-C). However, little is known about the impact of EL activity on plasma lipid profile. We aimed to establish a new method to evaluate EL-specific phospholipase activity in humans. Methods: Plasma samples were obtained from 115 patients with coronary artery disease(CAD) and 154 patients without CAD. Plasma EL protein was immunoprecipitated using an anti-EL monoclonal antibody after plasma non-specific immunoglobulins were removed by incubation with ProteinA. The phospholipase activity of the immunoprecipitated samples was measured using a fluorogenic phospholipase substrate, Bis-BODIPY FL C11-PC. Results: The EL-specific phospholipase assay revealed that plasma EL activity was inversely correlated with HDL-C levels(R=−0.3088, p＜0.0001). In addition, the EL activity was associated with cigarette smoking. Furthermore, EL activity in CAD patients was significantly higher than that in nonCAD patients. Concomitantly, the HDL-C level in CAD patients were significantly lower than that in non-CAD patients. Conclusion: We have established a method for human plasma EL-specific phospholipase activity by combination of EL immunoprecipitation and a fluorogenic phospholipid substrate. Plasma EL activity was associated with not only plasma HDL-C levels but also the risks for CAD.
ISSN:1340-3478
1880-3873
DOI:10.5551/jat.20131