Lysozyme binding to tethered bilayer lipid membranes prepared by rapid solvent exchange and vesicle fusion methods

• Published in: European biophysics journal 2014-05, Vol.43 (4-5), p.191-198
• Main Authors: Onaizi, Sagheer A., Nasser, M. S., Twaiq, Farouq
• Format: Article
• Language: English
• Subjects: Adsorption; Binding sites; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Biology; Cell Membrane - chemistry; Cell Membrane - metabolism; Comparative studies; Enzymes; Life Sciences; Lipid Bilayers - chemistry; Lipid Bilayers - metabolism; Lipids; Membrane Biology; Membrane Fusion; Membranes; Muramidase - chemistry; Muramidase - metabolism; Nanotechnology; Neurobiology; Original Paper; Protein Binding; Solvents; Solvents - chemistry
• ISSN: 0175-7571; 1432-1017
• DOI: 10.1007/s00249-014-0955-z

Tethered bilayer lipid membranes (tBLMs) are important tools for studying protein–lipid interactions. The widely used methodology for the preparation of these membranes is the fusion of phospholipid vesicles from an aqueous medium onto an anchored phospholipid layer. The preparation of phospholipid vesicles is a long and tedious procedure. There is another simple method, rapid solvent exchange, for preparing lipid membranes. However, there is a lack of information on the effects of the preparation method of tBLMs on their interactions with proteins. Therefore, we present in this paper a comparative study on the binding of lysozyme onto tBLMs prepared by the abovementioned methods. The prepared tBLMs have either zwitterionic or anionic characteristics. The results show that lysozyme binding onto the prepared tBLMs is unaffected by the preparation method of the tBLMs, suggesting that the tedious fusion method might be replaced by the simple rapid solvent exchange method without altering the level of protein–lipid interactions.