structure of particles of tobacco ringspot nepovirus: evidence from electron microscopy

Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, U.K. Particles of tobacco ringspot nepovirus from purified preparations were trapped on grids by immunosorbent electron microscopy and then either negatively stained, or freeze-dried and shadowed with uranium, for structural studies. Par...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 1988-08, Vol.69 (8), p.1831-1840
Main Author: Roberts, I.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
electron microscopy
Fundamental and applied biological sciences. Psychology
Microbiology
Morphology, structure, chemical composition, physicochemical properties
nepovirus
Nicotiana tabacum
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Systematics. Structure, properties and multiplication. Genetics
Tobacco ringspot virus
ultrastructure
Virology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, U.K. Particles of tobacco ringspot nepovirus from purified preparations were trapped on grids by immunosorbent electron microscopy and then either negatively stained, or freeze-dried and shadowed with uranium, for structural studies. Particle dimensions differed considerably with different stains and methods of preparation, but no obvious substructure was apparent. In contrast, particles which were freeze-dried and then shadowed exhibited either fivefold or threefold symmetry and had a structure resembling that of models made up of 60 units in clusters of five arranged in a T = 1 lattice. Both chemical and morphological evidence are compatible with this structure. Keywords: TRSV, structure, electron microscopy Received 5 April 1988; accepted 13 May 1988.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-69-8-1831