Down-regulation of WAVE2, WASP family verprolin-homologous protein 2, in gastric cancer indicates lymph node metastasis and cell migration

WAVE2 plays a crucial role in actin polymerisation and cell migration. We aimed to investigate the expression and cellular functions of WAVE2 in human gastric cancer (GC). The level of WAVE2 was determined using quantitative PCR (Q-PCR) in a cohort of human gastric tissues. Expression of WAVE2, ARP2...

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Bibliographic Details
Published in:Anticancer research 2014-05, Vol.34 (5), p.2185-2194
Main Authors: Jia, Shuqin, Jia, Yongning, Weeks, Hoi Ping, Ruge, Fiona, Feng, Xuemin, Ma, Ruiting, Ji, Jiafu, Ren, Jianjun, Jiang, Wen G
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Cell Movement - genetics
Down-Regulation
Epithelial-Mesenchymal Transition - genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Lymphatic Metastasis - genetics
Lymphatic Metastasis - pathology
Male
Middle Aged
Neoplasm Invasiveness - genetics
Neoplasm Invasiveness - pathology
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Transfection
Wiskott-Aldrich Syndrome Protein Family - biosynthesis
Young Adult
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Summary:WAVE2 plays a crucial role in actin polymerisation and cell migration. We aimed to investigate the expression and cellular functions of WAVE2 in human gastric cancer (GC). The level of WAVE2 was determined using quantitative PCR (Q-PCR) in a cohort of human gastric tissues. Expression of WAVE2, ARP2, NWASP, ROCK1 and ROCK2 was examined using RT-PCR in paired tissues. WAVE2 and ARP2 protein co-expression was examined. Anti-WAVE2 transgene ribozymes were constructed and transiently transfected into human GC cells. Down-regulation of WAVE2 expression in GC was significantly correlated with lymph node metastasis. WAVE2 was positively correlated with E-cadherin and negatively with TWIST. Immunohistochemically, WAVE2 and ARP2 were not co-expressed in serial mirror sections. In vitro, WAVE2 knockdown was shown to increase cell motility, whilst ROCK inhibitor treatment reduced this effect in HGC27 cells. WAVE2 is down-regulated in GC and loses its metastatic role in GC. Knockdown of WAVE2 could increase metastatic potential by promoting the growth, invasiveness, motility, adhesiveness and suppressing EMT (epithelial-mesenchymal transition) of GC cells.
ISSN:1791-7530