Global microRNA profiling of pancreatic neuroendocrine neoplasias

Pancreatic neuroendocrine neoplasms (pNEN) are rare tumors with a poor prognosis. Although increasing data have accumulated on the molecular pathology of pNEN, very scarce data exist on microRNAs in pNEN and no data are published on microRNAs as potential biomarkers of pNEN in serum. This study aime...

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Bibliographic Details
Published in:Anticancer research 2014-05, Vol.34 (5), p.2249-2254
Main Authors: Thorns, Chistoph, Schurmann, Claudia, Gebauer, Niklas, Wallaschofski, Henri, Kümpers, Christiane, Bernard, Veronica, Feller, Alfred C, Keck, Tobias, Habermann, Jens K, Begum, Nehara, Lehnert, Hendrik, Brabant, Georg
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - analysis
Biomarkers, Tumor - genetics
Female
Gene Expression Profiling - methods
Humans
Male
MicroRNAs - analysis
MicroRNAs - genetics
Middle Aged
Neuroendocrine Tumors - genetics
Oligonucleotide Array Sequence Analysis
Pancreatic Neoplasms - genetics
Real-Time Polymerase Chain Reaction
Transcriptome
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Summary:Pancreatic neuroendocrine neoplasms (pNEN) are rare tumors with a poor prognosis. Although increasing data have accumulated on the molecular pathology of pNEN, very scarce data exist on microRNAs in pNEN and no data are published on microRNAs as potential biomarkers of pNEN in serum. This study aimed to identify microRNA signatures of pNEN in tissue and serum. We included tissue samples from 37 patients with pNEN, 9 patients with non-neoplastic pancreatic pathology, seven samples of micro-dissected pancreatic islets and serum samples of 27 patients with pNEN, as well as of 15 healthy volunteers. MicroRNA expression profiles were established using real-time quantitative Polymerase Chain reaction (PCR) for 754 microRNAs. MicroRNA signatures differed between pNEN, pancreatic islets and total pancreas, with virtually no overlap between the groups of de-regulated microRNAs. Expression of miR-642 correlated with Ki67 (MiB1) score and miR-210 correlated with metastatic disease. When comparing microRNA levels in serum from patients with pNEN and healthy volunteers, 13 microRNAs were more abundant in the serum of patients. MiR-193b was also up-regulated in pNEN tissue when compared to pancreatic islets and remained significantly increased in serum even when corrected for multiple testing. Evaluation of microRNAs appears to be promising in the assessment of pNEN. In particular, miR-193b, which is also increased in serum, may be a potential new biomarker of pNEN.
ISSN:1791-7530