Microstructural Impact of Ischemia and Bone Marrow–Derived Cell Therapy Revealed With Diffusion Tensor Magnetic Resonance Imaging Tractography of the Heart In Vivo

• Published in: Circulation (New York, N.Y.) N.Y.), 2014-04, Vol.129 (17), p.1731-1741
• Main Authors: Sosnovik, David E, Mekkaoui, Choukri, Huang, Shuning, Chen, Howard H, Dai, Guangping, Stoeck, Christian T, Ngoy, Soeun, Guan, Jian, Wang, Ruopeng, Kostis, William J, Jackowski, Marcel P, Wedeen, Van J, Kozerke, Sebastian, Liao, Ronglih
• Format: Article
• Language: English
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Anisotropy; Applied cell therapy and gene therapy; Biological and medical sciences; Blood and lymphatic vessels; Bone Marrow Transplantation - methods; Bone marrow, stem cells transplantation. Graft versus host reaction; Cardiology. Vascular system; Diffusion Tensor Imaging - methods; Disease Models, Animal; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Healthy Volunteers; Imaging, Three-Dimensional - methods; Medical sciences; Mice; Mice, Inbred C57BL; Myocardial Infarction - pathology; Myocardial Infarction - therapy; Myocardial Ischemia - pathology; Myocardial Ischemia - therapy; Myocardium - pathology; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0009-7322; 1524-4539; 1524-4539
• DOI: 10.1161/CIRCULATIONAHA.113.005841

BACKGROUND—The arrangement of myofibers in the heart is highly complex and must be replicated by injected cells to produce functional myocardium. A novel approach to characterize the microstructural response of the myocardium to ischemia and cell therapy, with the use of serial diffusion tensor magnetic resonance imaging tractography of the heart in vivo, is presented. METHODS AND RESULTS—Validation of the approach was performed in normal (n=6) and infarcted mice (n=6) as well as healthy human volunteers. Mice (n=12) were then injected with bone marrow mononuclear cells 3 weeks after coronary ligation. In half of the mice the donor and recipient strains were identical, and in half the strains were different. A positive response to cell injection was defined by a decrease in mean diffusivity, an increase in fractional anisotropy, and the appearance of new myofiber tracts with the correct orientation. A positive response to bone marrow mononuclear cell injection was seen in 1 mouse. The response of the majority of mice to bone marrow mononuclear cell injection was neutral (9/12) or negative (2/12). The in vivo tractography findings were confirmed with histology. CONCLUSIONS—Diffusion tensor magnetic resonance imaging tractography was able to directly resolve the ability of injected cells to generate new myofiber tracts and provided a fundamental readout of their regenerative capacity. A highly novel and translatable approach to assess the efficacy of cell therapy in the heart is thus presented.