1,3- beta -glucanases from Streptomyces sp.1228 lytic enzyme system

Four 1,3- beta -glucanases GI, GII, GIV and GVIII from a culture filtrate of Streptomyces sp.1228 were purified by anion exchange chromatography using DEAE-Sepharose Cl-6B or DEAE-Cellulose, gel filtration on Bio-Gel P-200 or Sephacryl S- 200, Amicon ultrafiltration and preparative PAGE. The M sub(r...

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Bibliographic Details
Published in:Biotechnology letters 1989-01, Vol.11 (4), p.281-286
Main Authors: Bielecki, S, Wnuk, M, Szczesna, M, Bobowicz-Lassocinska, T, Antczak, T, Galas, E
Format: Article
Language:English
Subjects:
1,3- beta -glucanase
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Summary:Four 1,3- beta -glucanases GI, GII, GIV and GVIII from a culture filtrate of Streptomyces sp.1228 were purified by anion exchange chromatography using DEAE-Sepharose Cl-6B or DEAE-Cellulose, gel filtration on Bio-Gel P-200 or Sephacryl S- 200, Amicon ultrafiltration and preparative PAGE. The M sub(r) of these enzymes were 19,000, 74,000, 78,000 and 56,000 respectively. The glucanase GVIII consisted of two subunits. The optimal catalytic activity of the purified preparations was at 50-55 degree C and pH 5.5 - 6.0. The enzymes were also most stable at this pH. Both glucanases GI and GVIII were characterized by high thermostability.
ISSN:0141-5492
DOI:10.1007/BF01031578