miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary

During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes...

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Bibliographic Details
Published in:Genetics and molecular research 2014-01, Vol.13 (2), p.2504-2512
Main Authors: Tu, F, Pan, Z X, Yao, Y, Liu, H L, Liu, S R, Xie, Z, Li, Q F
Format: Article
Language:English
Subjects:
Animals
Apoptosis - genetics
Female
Flow Cytometry
Granulosa Cells - pathology
Inhibin-beta Subunits - genetics
Inhibin-beta Subunits - metabolism
MicroRNAs - genetics
Ovarian Follicle - growth & development
Ovarian Follicle - metabolism
Ovary - metabolism
Sus scrofa
Swine
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Summary:During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in pig ovarian follicles. In addition, inhibin beta B was found to be a miR-34a target gene, based on luciferase reporter assays, quantitative RT-PCR and Western blotting. Taken together, our data indicate that miR-34a plays an important role in granulosa cell apoptosis by targeting the INHBB gene in the porcine ovary.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.january.14.6