Quantification analysis of yeast-displayed lipase

We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as “g/g cell” or “molecules/cell”. T...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 2014-04, Vol.450, p.46-48
Main Authors: Jin, Zi, Zhang, Kun, Zhang, Li, Zheng, Sui-Ping, Han, Shuang-Yan, Lin, Ying
Format: Article
Language:English
Subjects:
Active-site titration
Cell Surface Display Techniques - methods
Enzyme Inhibitors - pharmacology
Fungal Proteins - antagonists & inhibitors
Fungal Proteins - genetics
Lipase
Lipase - antagonists & inhibitors
Lipase - genetics
Organophosphonates - pharmacology
Pichia - genetics
Quantification
Saccharomyces cerevisiae - cytology
Yeast surface display
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as “g/g cell” or “molecules/cell”. The results obtained correlated well with those obtained by existing methods. Therefore, this method is credible and will provide a powerful tool to promote research of lipase yeast surface display.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2013.12.035