Quantification analysis of yeast-displayed lipase

We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as “g/g cell” or “molecules/cell”. T...

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Published in:Analytical biochemistry 2014-04, Vol.450, p.46-48
Main Authors: Jin, Zi, Zhang, Kun, Zhang, Li, Zheng, Sui-Ping, Han, Shuang-Yan, Lin, Ying
Format: Article
Language:English
Subjects:
Active-site titration
Cell Surface Display Techniques - methods
Enzyme Inhibitors - pharmacology
Fungal Proteins - antagonists & inhibitors
Fungal Proteins - genetics
Lipase
Lipase - antagonists & inhibitors
Lipase - genetics
Organophosphonates - pharmacology
Pichia - genetics
Quantification
Saccharomyces cerevisiae - cytology
Yeast surface display
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cited_by cdi_FETCH-LOGICAL-c383t-a5810ec79cfe857c8b7fd275674f84ed8703241339b68d54f21ef9e4273044a73
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container_start_page 46
container_title Analytical biochemistry
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creator Jin, Zi
Zhang, Kun
Zhang, Li
Zheng, Sui-Ping
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description We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as “g/g cell” or “molecules/cell”. The results obtained correlated well with those obtained by existing methods. Therefore, this method is credible and will provide a powerful tool to promote research of lipase yeast surface display.
doi_str_mv 10.1016/j.ab.2013.12.035
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subjects Active-site titration
Cell Surface Display Techniques - methods
Enzyme Inhibitors - pharmacology
Fungal Proteins - antagonists & inhibitors
Fungal Proteins - genetics
Lipase
Lipase - antagonists & inhibitors
Lipase - genetics
Organophosphonates - pharmacology
Pichia - genetics
Quantification
Saccharomyces cerevisiae - cytology
Yeast surface display
title Quantification analysis of yeast-displayed lipase
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