First report of Enterocytozoon bieneusi from dairy cattle in Argentina

Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14...

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Bibliographic Details
Published in:Veterinary parasitology 2014-01, Vol.199 (1-2), p.112-115
Main Authors: Del Coco, Valeria F., Córdoba, María Alejandra, Bilbao, Gladys, de Almeida Castro, Pinto, Basualdo, Juan Angel, Santín, Mónica
Format: Article
Language:English
Subjects:
Animals
Argentina
Argentina - epidemiology
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - microbiology
Dairying
DNA, Ribosomal Spacer - genetics
Enterocytozoon - genetics
Enterocytozoon - isolation & purification
Enterocytozoon - physiology
Enterocytozoon bieneusi
Feces - microbiology
Genotype
Humans
Microsporidia
Microsporidiosis - epidemiology
Microsporidiosis - microbiology
Microsporidiosis - veterinary
Molecular Sequence Data
PCR
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Summary:Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2013.09.024