DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor

MicroRNAs (miRNAs) are key regulators of a wide range of cellular processes, and have been identified as promising cancer biomarkers due to their stable presence in serum. As an surface-based electrochemical biosensors which offer great opportunities for low-cost, point-of-care tests (POCTs) of dise...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2013-12, Vol.64 (3), p.276-282
Main Authors: Wen, Yanli, Liu, Gang, Pei, Hao, Li, Lanying, Xu, Qin, Liang, Wen, Li, Yan, Xu, Li, Ren, Suzhen, Fan, Chunhai
Format: Article
Language:English
Subjects:
Biosensing Techniques
Carcinoma, Squamous Cell - metabolism
DNA nanostructure
DNA Probes - chemistry
Electrochemical DNA sensors
Electrochemical Techniques
Enzyme amplification
Esophageal Neoplasms - metabolism
Humans
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Nucleic Acid Conformation
Nucleic Acid Hybridization
Sensitivity and Specificity
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Summary:MicroRNAs (miRNAs) are key regulators of a wide range of cellular processes, and have been identified as promising cancer biomarkers due to their stable presence in serum. As an surface-based electrochemical biosensors which offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. Nevertheless, the sensitivity of miRNA sensors is often limited by mass transport and the surface crowding effect at the water-electrode interface. Here, we present a protocol as well as guidelines for ultrasensitive detection of miRNA with DNA nanostructure-based electrochemical miRNA biosensor. By employing the three-dimensional DNA nanostructure-based interfacial engineering approach, we can directly detect as few as attomolar (
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2013.07.035