An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequence-specific DNA-binding protein

In this paper, we report for the first time an electrochemical biosensor for single-step, reagentless, and picomolar detection of a sequence-specific DNA-binding protein using a double-stranded, electrode-bound DNA probe terminally modified with a redox active label close to the electrode surface. T...

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Bibliographic Details
Published in:Analyst (London) 2014-05, Vol.139 (9), p.2193-2198
Main Authors: Zhang, Yun, Liu, Fang, Nie, Jinfang, Jiang, Fuyang, Zhou, Caibin, Yang, Jiani, Fan, Jinlong, Li, Jianping
Format: Article
Language:English
Subjects:
Diffusion
DNA-Binding Proteins - chemistry
Electrochemical Techniques - instrumentation
Electrolytes - chemistry
Indicators and Reagents - chemistry
Limit of Detection
Reproducibility of Results
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Summary:In this paper, we report for the first time an electrochemical biosensor for single-step, reagentless, and picomolar detection of a sequence-specific DNA-binding protein using a double-stranded, electrode-bound DNA probe terminally modified with a redox active label close to the electrode surface. This new methodology is based upon local repression of electrolyte diffusion associated with protein-DNA binding that leads to reduction of the electrochemical response of the label. In the proof-of-concept study, the resulting electrochemical biosensor was quantitatively sensitive to the concentrations of the TATA binding protein (TBP, a model analyte) ranging from 40 pM to 25.4 nM with an estimated detection limit of ∼10.6 pM (∼80 to 400-fold improvement on the detection limit over previous electrochemical analytical systems).
ISSN:0003-2654
1364-5528
DOI:10.1039/c4an00096j