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Mechanical processing of hyperviscous semen specimens can negatively affect sperm DNA fragmentation
Introduction The present study compared the DNA fragmentation in human sperm samples with reduced, physiological, and increased viscosity in order to evaluate whether the process used to reduce viscosity (expulsion of semen through a needle and syringe) alters significantly sperm DNA fragmentation....
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| Published in: | International urology and nephrology 2014-04, Vol.46 (4), p.737-742 |
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| container_title | International urology and nephrology |
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| creator | Kussler, Ana Paula S. Pimentel, Anita M. Alcoba, Diego D. Liu, Isabella P. Brum, Ilma Simoni Capp, Edison Corleta, Helena V. E. |
| description | Introduction
The present study compared the DNA fragmentation in human sperm samples with reduced, physiological, and increased viscosity in order to evaluate whether the process used to reduce viscosity (expulsion of semen through a needle and syringe) alters significantly sperm DNA fragmentation.
Methods
The seminal parameters of semen samples from 123 patients were evaluated and classified according to their viscosity. Samples with increased viscosity were submitted to a process of expulsion of semen through a 10-mL syringe and an 18-gauge (18G) needle to reduce the seminal viscosity. The DNA fragmentation of all samples was analysed using TUNEL assay (Terminal deoxynucleotidyl transferase mediated dUTP Nick-end labelling assay); in samples with increased viscosity, the fragmentation was assessed before and after the process of expulsion with syringe and needle.
Results
There was no difference in DNA fragmentation between groups with different viscosity (
P
= 0.857). A significantly increase in sperm DNA fragmentation after expulsion of hyperviscous semen through the syringe was observed (
P
= 0.035).
Conclusion
There was no difference in DNA fragmentation rate between samples with reduced, increased and physiological viscosities; however, the physical process of expulsion of semen through a syringe and needle increased sperm DNA fragmentation. |
| doi_str_mv | 10.1007/s11255-013-0578-9 |
| format | article |
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The present study compared the DNA fragmentation in human sperm samples with reduced, physiological, and increased viscosity in order to evaluate whether the process used to reduce viscosity (expulsion of semen through a needle and syringe) alters significantly sperm DNA fragmentation.
Methods
The seminal parameters of semen samples from 123 patients were evaluated and classified according to their viscosity. Samples with increased viscosity were submitted to a process of expulsion of semen through a 10-mL syringe and an 18-gauge (18G) needle to reduce the seminal viscosity. The DNA fragmentation of all samples was analysed using TUNEL assay (Terminal deoxynucleotidyl transferase mediated dUTP Nick-end labelling assay); in samples with increased viscosity, the fragmentation was assessed before and after the process of expulsion with syringe and needle.
Results
There was no difference in DNA fragmentation between groups with different viscosity (
P
= 0.857). A significantly increase in sperm DNA fragmentation after expulsion of hyperviscous semen through the syringe was observed (
P
= 0.035).
Conclusion
There was no difference in DNA fragmentation rate between samples with reduced, increased and physiological viscosities; however, the physical process of expulsion of semen through a syringe and needle increased sperm DNA fragmentation.</description><identifier>ISSN: 0301-1623</identifier><identifier>EISSN: 1573-2584</identifier><identifier>DOI: 10.1007/s11255-013-0578-9</identifier><identifier>PMID: 24096372</identifier><identifier>CODEN: IURNAE</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adult ; Cross-Sectional Studies ; DNA Fragmentation ; Humans ; In Situ Nick-End Labeling ; Male ; Mechanical Phenomena ; Medicine ; Medicine & Public Health ; Nephrology ; Prospective Studies ; Semen ; Specimen Handling - adverse effects ; Spermatozoa - physiology ; Urology ; Urology - Original Paper ; Viscosity</subject><ispartof>International urology and nephrology, 2014-04, Vol.46 (4), p.737-742</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-4a4e56bd789a5ca1d28bb80dfc09d3554f79460527967b4d1ac1f2d9864929d63</citedby><cites>FETCH-LOGICAL-c405t-4a4e56bd789a5ca1d28bb80dfc09d3554f79460527967b4d1ac1f2d9864929d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24096372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kussler, Ana Paula S.</creatorcontrib><creatorcontrib>Pimentel, Anita M.</creatorcontrib><creatorcontrib>Alcoba, Diego D.</creatorcontrib><creatorcontrib>Liu, Isabella P.</creatorcontrib><creatorcontrib>Brum, Ilma Simoni</creatorcontrib><creatorcontrib>Capp, Edison</creatorcontrib><creatorcontrib>Corleta, Helena V. E.</creatorcontrib><title>Mechanical processing of hyperviscous semen specimens can negatively affect sperm DNA fragmentation</title><title>International urology and nephrology</title><addtitle>Int Urol Nephrol</addtitle><addtitle>Int Urol Nephrol</addtitle><description>Introduction
The present study compared the DNA fragmentation in human sperm samples with reduced, physiological, and increased viscosity in order to evaluate whether the process used to reduce viscosity (expulsion of semen through a needle and syringe) alters significantly sperm DNA fragmentation.
Methods
The seminal parameters of semen samples from 123 patients were evaluated and classified according to their viscosity. Samples with increased viscosity were submitted to a process of expulsion of semen through a 10-mL syringe and an 18-gauge (18G) needle to reduce the seminal viscosity. The DNA fragmentation of all samples was analysed using TUNEL assay (Terminal deoxynucleotidyl transferase mediated dUTP Nick-end labelling assay); in samples with increased viscosity, the fragmentation was assessed before and after the process of expulsion with syringe and needle.
Results
There was no difference in DNA fragmentation between groups with different viscosity (
P
= 0.857). A significantly increase in sperm DNA fragmentation after expulsion of hyperviscous semen through the syringe was observed (
P
= 0.035).
Conclusion
There was no difference in DNA fragmentation rate between samples with reduced, increased and physiological viscosities; however, the physical process of expulsion of semen through a syringe and needle increased sperm DNA fragmentation.</description><subject>Adult</subject><subject>Cross-Sectional Studies</subject><subject>DNA Fragmentation</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Mechanical Phenomena</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nephrology</subject><subject>Prospective Studies</subject><subject>Semen</subject><subject>Specimen Handling - adverse effects</subject><subject>Spermatozoa - physiology</subject><subject>Urology</subject><subject>Urology - Original Paper</subject><subject>Viscosity</subject><issn>0301-1623</issn><issn>1573-2584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkU1rFTEUhoMo9lr9AW4k4MbN2HPyMUmWpfULWrup65DJJLdTZjLXZG7h_nsz3ioiCK4SOM95k5eHkNcI7xFAnRVEJmUDyBuQSjfmCdmgVLxhUounZAMcsMGW8RPyopR7ADAa4Dk5YQJMyxXbEH8d_J1Lg3cj3eXZh1KGtKVzpHeHXcgPQ_HzvtASppBo2QU_1Euh3iWawtYtw0MYD9TFGPyyzvNEL7-e05jdtoJLBeb0kjyLbizh1eN5Sr59_HB78bm5uvn05eL8qvEC5NIIJ4Jsu15p46R32DPddRr66MH0XEoRlREtSKZMqzrRo_MYWW90KwwzfctPybtjbi3yfR_KYqf6_TCOLoVawqJkwJUyHP8DRdQMFa6pb_9C7-d9TrXITwo0Mm4qhUfK57mUHKLd5WFy-WAR7CrLHmXZKsuusuy68-Yxed9Nof-98ctOBdgRKHWUtiH_8fQ_U38A46Oepw</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Kussler, Ana Paula S.</creator><creator>Pimentel, Anita M.</creator><creator>Alcoba, Diego D.</creator><creator>Liu, Isabella P.</creator><creator>Brum, Ilma Simoni</creator><creator>Capp, Edison</creator><creator>Corleta, Helena V. 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E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-4a4e56bd789a5ca1d28bb80dfc09d3554f79460527967b4d1ac1f2d9864929d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Cross-Sectional Studies</topic><topic>DNA Fragmentation</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Mechanical Phenomena</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nephrology</topic><topic>Prospective Studies</topic><topic>Semen</topic><topic>Specimen Handling - adverse effects</topic><topic>Spermatozoa - physiology</topic><topic>Urology</topic><topic>Urology - Original Paper</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kussler, Ana Paula S.</creatorcontrib><creatorcontrib>Pimentel, Anita M.</creatorcontrib><creatorcontrib>Alcoba, Diego D.</creatorcontrib><creatorcontrib>Liu, Isabella P.</creatorcontrib><creatorcontrib>Brum, Ilma Simoni</creatorcontrib><creatorcontrib>Capp, Edison</creatorcontrib><creatorcontrib>Corleta, Helena V. E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>International urology and nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kussler, Ana Paula S.</au><au>Pimentel, Anita M.</au><au>Alcoba, Diego D.</au><au>Liu, Isabella P.</au><au>Brum, Ilma Simoni</au><au>Capp, Edison</au><au>Corleta, Helena V. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical processing of hyperviscous semen specimens can negatively affect sperm DNA fragmentation</atitle><jtitle>International urology and nephrology</jtitle><stitle>Int Urol Nephrol</stitle><addtitle>Int Urol Nephrol</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>46</volume><issue>4</issue><spage>737</spage><epage>742</epage><pages>737-742</pages><issn>0301-1623</issn><eissn>1573-2584</eissn><coden>IURNAE</coden><abstract>Introduction
The present study compared the DNA fragmentation in human sperm samples with reduced, physiological, and increased viscosity in order to evaluate whether the process used to reduce viscosity (expulsion of semen through a needle and syringe) alters significantly sperm DNA fragmentation.
Methods
The seminal parameters of semen samples from 123 patients were evaluated and classified according to their viscosity. Samples with increased viscosity were submitted to a process of expulsion of semen through a 10-mL syringe and an 18-gauge (18G) needle to reduce the seminal viscosity. The DNA fragmentation of all samples was analysed using TUNEL assay (Terminal deoxynucleotidyl transferase mediated dUTP Nick-end labelling assay); in samples with increased viscosity, the fragmentation was assessed before and after the process of expulsion with syringe and needle.
Results
There was no difference in DNA fragmentation between groups with different viscosity (
P
= 0.857). A significantly increase in sperm DNA fragmentation after expulsion of hyperviscous semen through the syringe was observed (
P
= 0.035).
Conclusion
There was no difference in DNA fragmentation rate between samples with reduced, increased and physiological viscosities; however, the physical process of expulsion of semen through a syringe and needle increased sperm DNA fragmentation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24096372</pmid><doi>10.1007/s11255-013-0578-9</doi><tpages>6</tpages></addata></record> |
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| ispartof | International urology and nephrology, 2014-04, Vol.46 (4), p.737-742 |
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| language | eng |
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| source | Springer Nature |
| subjects | Adult Cross-Sectional Studies DNA Fragmentation Humans In Situ Nick-End Labeling Male Mechanical Phenomena Medicine Medicine & Public Health Nephrology Prospective Studies Semen Specimen Handling - adverse effects Spermatozoa - physiology Urology Urology - Original Paper Viscosity |
| title | Mechanical processing of hyperviscous semen specimens can negatively affect sperm DNA fragmentation |
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