Comparison of two biochemical methods for identifying Corynebacterium pseudotuberculosis isolated from sheep and goats

The biochemical pattern of Cowan and Steel (BPCS) was compared with a commercial biochemical strip for the identification of Corynebacterium pseudotuberculosis isolated from small ruminants. On 16S rRNA gene sequencing, 40/78 coryneform isolates from the lymph nodes of sheep and goats with lesions r...

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Bibliographic Details
Published in:The veterinary journal (1997) 2013-06, Vol.196 (3), p.552-554
Main Authors: Huerta, Belén, Gómez-Gascón, Lidia, Vela, Ana I., Fernández-Garayzábal, José F., Casamayor, Almudena, Tarradas, Carmen, Maldonado, Alfonso
Format: Article
Language:English
Subjects:
16S rRNA gene sequencing
Animals
Biochemical identification
caseous lymphadenitis
Corynebacterium Infections - diagnosis
Corynebacterium Infections - microbiology
Corynebacterium Infections - veterinary
Corynebacterium pseudotuberculosis
Corynebacterium pseudotuberculosis - isolation & purification
genes
Goat Diseases - diagnosis
Goat Diseases - microbiology
Goats
lymph nodes
microbiology
nucleotide sequences
Reagent Strips
ribosomal RNA
RNA, Bacterial - classification
RNA, Bacterial - genetics
RNA, Bacterial - isolation & purification
RNA, Ribosomal, 16S
Ruminantia
Sensitivity and Specificity
Sheep
Sheep Diseases - diagnosis
Sheep Diseases - microbiology
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Summary:The biochemical pattern of Cowan and Steel (BPCS) was compared with a commercial biochemical strip for the identification of Corynebacterium pseudotuberculosis isolated from small ruminants. On 16S rRNA gene sequencing, 40/78 coryneform isolates from the lymph nodes of sheep and goats with lesions resembling caseous lymphadenitis were identified as C. pseudotuberculosis. The sensitivities of the BPCS and the commercial biochemical strip relative to 16S rRNA sequencing were 80% and 85%, and their specificities were 92.1% and 94.7%, respectively; the level of agreement between the BPCS and the commercial biochemical strip was high (κ=0.82). Likelihood ratios for positive and negative results were 10.0 and 0.22 for the BPCS, and 16.0 and 0.16 for the commercial biochemical strip, respectively. These results indicate that the BPCS and the commercial biochemical strip are both useful for identifying C. pseudotuberculosis in veterinary microbiology laboratories.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2012.12.008