Attenuation of Theileria lestoquardi infected cells and immunization of sheep against malignant ovine theileriosis

Abstract Malignant ovine theileriosis caused by Theileria lestoquardi is an economically important disease infecting small ruminants in the Sudan. The disease causes massive losses among sheep in many regions of Northern Sudan. The present studies were done to isolate lymphoblastiod cells infected w...

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Bibliographic Details
Published in:Vaccine 2013-10, Vol.31 (42), p.4775-4781
Main Authors: Ahmed, Bukhari M, Taha, Khalid M, Enan, Khalid A, Elfahal, Abdelghafar M, El Hussein, Abdel Rahim M
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Applied microbiology
Attenuation
Biological and medical sciences
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Immunization
Immunization - methods
Medical sciences
Microbiology
Parasitemia - parasitology
Parasitemia - pathology
Parasitemia - prevention & control
Parasitemia - veterinary
Protozoan Vaccines - administration & dosage
Protozoan Vaccines - adverse effects
Protozoan Vaccines - immunology
Ruminantia
Sheep
Sheep Diseases - parasitology
Sheep Diseases - pathology
Sheep Diseases - prevention & control
Sudan
Survival Analysis
Theileria - immunology
Theileria lestoquardi
Theileriasis - parasitology
Theileriasis - pathology
Theileriasis - prevention & control
Tumors
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Attenuated - administration & dosage
Vaccines, Attenuated - adverse effects
Vaccines, Attenuated - immunology
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Summary:Abstract Malignant ovine theileriosis caused by Theileria lestoquardi is an economically important disease infecting small ruminants in the Sudan. The disease causes massive losses among sheep in many regions of Northern Sudan. The present studies were done to isolate lymphoblastiod cells infected with malignant ovine theileriosis and attenuate them by passage using culture media to develop and produce schizonts candidate vaccine, then test its efficacy and safety by exposing immunized lambs to field challenge in an area endemic with T. lestoquardi . In the present experiments we isolated and established an in vitro culture of T. lestoquardi infected lymphoblast cell line. Long-term culture of T. lestoquardi infected lymphoplastoid cells was shown to result in attenuation of their virulence and lambs inoculated with different doses of such cells at passage 105 exhibited very mild reactions with fever that lasted for 1–5 days and parasitaemia of
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2013.08.004