Newcastle Disease Virus (NDV) Iraqi Strain AD2141 Induces DNA Damage and FasL in Cancer Cell Lines

The oncolytic viruses are promising form of cancer therapy which is based on the selectively killing of the cancer cells. This study aimed to investigate the role of Newcastle disease virus Iraqi strain AD2141 in apoptosis. Firstly, the virulence of AD2141 was detected in embryonated chicken eggs af...

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Bibliographic Details
Published in:Journal of biology and life science 2014-01, Vol.5 (1), p.1-1
Main Authors: Al-Shammary, Ahmed M, Hassani, Hayfa H, Ibrahim, Ula A J
Format: Article
Language:English
Subjects:
Newcastle disease virus
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Summary:The oncolytic viruses are promising form of cancer therapy which is based on the selectively killing of the cancer cells. This study aimed to investigate the role of Newcastle disease virus Iraqi strain AD2141 in apoptosis. Firstly, the virulence of AD2141 was detected in embryonated chicken eggs after 48hrs of infection. It was observed a hemorrhage in the skin of infected embryos that led to death. By using cytotoxicity test, it was found 128 HAU/ml of AD2141 had a potent inhibition against growth of RD and AMN3 after 72hrs of exposure time; the inhibition rate was 86.8% and 86.98% respectively. Moreover, the apoptotic activity of AD2141 was examined by comet assay. A significant induction of DNA damage in cancer cells was determined after 48hrs of exposure time; the average DNA tail length in RD, AMN3, and AMGM was 55.46, 79.1, and 84.4 respectively, and the percentage of apoptosis in treated RD, AMN-3, and AMGM was 78%, 92%, and 94% respectively.
ISSN:2157-6076