Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646

• Published in: Bioresource technology 2013-10, Vol.146, p.215-222
• Main Authors: Volova, Tatiana G., Kiselev, Evgeniy G., Shishatskaya, Ekaterina I., Zhila, Natalia O., Boyandin, Anatoly N., Syrvacheva, Daria A., Vinogradova, Olga N., Kalacheva, Galina S., Vasiliev, Alexander D., Peterson, Ivan V.
• Format: Article
• Language: English
• Subjects: 3-Hydroxybutyric Acid - chemistry; Autotrophic synthesis; Biodegradation, Environmental; Bioreactors; Carbon; Carbon - chemistry; Carbon dioxide; Carbon Dioxide - chemistry; Clostridium beijerinckii - metabolism; Constraining; Crystallization; Culture; Cupriavidus eutrophus; Fermentation; Gases; Hydrogen - chemistry; Mass transfer; Monomers; Oxygen - chemistry; Polyhydroxyalkanoates; Polyhydroxyalkanoates - chemistry; Polymers - chemistry; Refuse Disposal - methods; Sulfur; Temperature
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.07.070

•PHA synthesis by Cupriavidus eutrophus В-10646 was studied in autotrophic culture.•Conditions of the synthesis of PHAs with different composition have been found.•Physicochemical properties of different PHA specimens have been studied. Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus – Cupriavidus eutrophus B-10646 – was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h−1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h−1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, γ-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties.