Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum

• Published in: International journal of paediatric dentistry 2014-05, Vol.24 (3), p.226-233
• Main Authors: Yang, Ning-Yan, Zhang, Quan, Li, Jin-Lu, Yang, Sheng-Hui, Shi, Qing
• Format: Article
• Language: English
• Subjects: Adolescent; Child; Children & youth; Colony Count, Microbial; Dental Plaque - microbiology; Dentistry; Disease Progression; Female; Fusobacterium nucleatum; Fusobacterium nucleatum - isolation & purification; Gum disease; Humans; Male; Molecular Sequence Data; Periodontitis - microbiology; Periodontitis - physiopathology; Porphyromonas gingivalis; Porphyromonas gingivalis - isolation & purification; Prevotella intermedia; Prevotella intermedia - isolation & purification
• ISSN: 0960-7439; 1365-263X
• DOI: 10.1111/ipd.12065

Objective The study aims to evaluate the change of related subgingival periodontopathogens among different stage of gingivitis in adolescent and assess the relationship between periodontopathogens and the progression of periodontal inflammation. Methods A total of 77 subgingival plaque samples from 35 adolescent individuals were divided into three groups including gingivitis group (mild, 15 samples; moderate, 16 samples; severe, 15 samples), chronic periodontitis group (15 samples) and healthy group (15 samples). Real‐time PCR was used to quantitate Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum in subgingival plaque samples. Results All species, except for F. nucleatum, were detected in samples from gingivitis and periodontitis groups in significantly greater number than in those from healthy group (P