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Application of airlift bioreactors to accelerate genetic transformation in American chestnut
Airlift bioreactors (ALBs) were constructed and tested for their potential to enhance chestnut embryogenic tissue proliferation for genetic transformation and mass propagation. Multiple genotypes of American chestnut ( Castanea dentata ) and backcross hybrids of American chestnut and Chinese chestnu...
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| Published in: | Plant cell, tissue and organ culture tissue and organ culture, 2014-04, Vol.117 (1), p.39-50 |
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| container_start_page | 39 |
| container_title | Plant cell, tissue and organ culture |
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| creator | Kong, Lisheng Holtz, Christine T. Nairn, Campbell J. Houke, Haley Powell, William A. Baier, Kathleen Merkle, Scott A. |
| description | Airlift bioreactors (ALBs) were constructed and tested for their potential to enhance chestnut embryogenic tissue proliferation for genetic transformation and mass propagation. Multiple genotypes of American chestnut (
Castanea dentata
) and backcross hybrids of American chestnut and Chinese chestnut (
C. dentata
×
Castanea mollissima
) were cultured in ALBs. Medium for tissue proliferation in ALBs was woody plant medium supplemented with 3 g l
−1
sucrose, 0.5 g l
−1
l
-glutamine and 2 mg l
−1
2,4-dichlorophenoxyacetic acid. Optimum culture conditions for 1-l ALBs included 2 % (w/v) tissue inoculation density for culture initiation, 200 ml min
−1
airflow rate, and weekly feeding with fresh medium (80–85 %, v/v). Compared with flasks, ALBs generated higher yields of tissue mass and larger fractions of small cell clumps ( |
| doi_str_mv | 10.1007/s11240-013-0418-8 |
| format | article |
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Castanea dentata
) and backcross hybrids of American chestnut and Chinese chestnut (
C. dentata
×
Castanea mollissima
) were cultured in ALBs. Medium for tissue proliferation in ALBs was woody plant medium supplemented with 3 g l
−1
sucrose, 0.5 g l
−1
l
-glutamine and 2 mg l
−1
2,4-dichlorophenoxyacetic acid. Optimum culture conditions for 1-l ALBs included 2 % (w/v) tissue inoculation density for culture initiation, 200 ml min
−1
airflow rate, and weekly feeding with fresh medium (80–85 %, v/v). Compared with flasks, ALBs generated higher yields of tissue mass and larger fractions of small cell clumps (<1 mm in diameter) that were optimum target material for transformation. Bioreactor-produced tissue demonstrated high amenability to transformation with reporter genes and chestnut blight resistance candidate genes (CGs) via
Agrobacterium
co-cultivation. The thaumatin-like protein CG was transformed effectively into ALB-produced target material of four genotypes, resulting in thousands of G418-resistant cell clumps, or transgenic events, which reached up to 70 % of the total target cell clumps in liquid selection medium for 3–6 weeks. Substantial acceleration of chestnut transformation was achieved with ALB technology, by which larger quantities of high quality target tissue was produced at lower labor and operating expense than previously used approaches.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-013-0418-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acceleration ; Agrobacterium ; Air flow ; Biomedical and Life Sciences ; Bioreactors ; Blight ; Castanea dentata ; Castanea mollissima ; Cell culture ; Chestnut ; Clumps ; Cultivation ; Dichlorophenoxyacetic acid ; Flasks ; Genes ; Genetic transformation ; Genotypes ; Glutamine ; Hybrids ; Inoculation ; Life Sciences ; Original Paper ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Propagation ; Sucrose ; Sugar ; Thaumatin ; Tissue culture ; Tissues ; Woody plants</subject><ispartof>Plant cell, tissue and organ culture, 2014-04, Vol.117 (1), p.39-50</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2013). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-df625e2ddb45e1f95c417ce3b0c47bc871383bfe3016a371be68623b3ab95b6b3</citedby><cites>FETCH-LOGICAL-c349t-df625e2ddb45e1f95c417ce3b0c47bc871383bfe3016a371be68623b3ab95b6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Kong, Lisheng</creatorcontrib><creatorcontrib>Holtz, Christine T.</creatorcontrib><creatorcontrib>Nairn, Campbell J.</creatorcontrib><creatorcontrib>Houke, Haley</creatorcontrib><creatorcontrib>Powell, William A.</creatorcontrib><creatorcontrib>Baier, Kathleen</creatorcontrib><creatorcontrib>Merkle, Scott A.</creatorcontrib><title>Application of airlift bioreactors to accelerate genetic transformation in American chestnut</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Airlift bioreactors (ALBs) were constructed and tested for their potential to enhance chestnut embryogenic tissue proliferation for genetic transformation and mass propagation. Multiple genotypes of American chestnut (
Castanea dentata
) and backcross hybrids of American chestnut and Chinese chestnut (
C. dentata
×
Castanea mollissima
) were cultured in ALBs. Medium for tissue proliferation in ALBs was woody plant medium supplemented with 3 g l
−1
sucrose, 0.5 g l
−1
l
-glutamine and 2 mg l
−1
2,4-dichlorophenoxyacetic acid. Optimum culture conditions for 1-l ALBs included 2 % (w/v) tissue inoculation density for culture initiation, 200 ml min
−1
airflow rate, and weekly feeding with fresh medium (80–85 %, v/v). Compared with flasks, ALBs generated higher yields of tissue mass and larger fractions of small cell clumps (<1 mm in diameter) that were optimum target material for transformation. Bioreactor-produced tissue demonstrated high amenability to transformation with reporter genes and chestnut blight resistance candidate genes (CGs) via
Agrobacterium
co-cultivation. The thaumatin-like protein CG was transformed effectively into ALB-produced target material of four genotypes, resulting in thousands of G418-resistant cell clumps, or transgenic events, which reached up to 70 % of the total target cell clumps in liquid selection medium for 3–6 weeks. Substantial acceleration of chestnut transformation was achieved with ALB technology, by which larger quantities of high quality target tissue was produced at lower labor and operating expense than previously used approaches.</description><subject>Acceleration</subject><subject>Agrobacterium</subject><subject>Air flow</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Blight</subject><subject>Castanea dentata</subject><subject>Castanea mollissima</subject><subject>Cell culture</subject><subject>Chestnut</subject><subject>Clumps</subject><subject>Cultivation</subject><subject>Dichlorophenoxyacetic acid</subject><subject>Flasks</subject><subject>Genes</subject><subject>Genetic transformation</subject><subject>Genotypes</subject><subject>Glutamine</subject><subject>Hybrids</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Propagation</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Thaumatin</subject><subject>Tissue culture</subject><subject>Tissues</subject><subject>Woody plants</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp1kMtKAzEUhoMoWC8P4C7gxs1orjPpshRvUHCjOyEk6ZmaMk1qkln49qaMIAiuzub7_3POh9AVJbeUkO4uU8oEaQjlDRFUNeoIzajseCOJEMdoRmjbNa2S3Sk6y3lLCGm5oDP0vtjvB-9M8THg2GPj0-D7gq2PCYwrMWVcIjbOwQDJFMAbCFC8wyWZkPuYdlPWB7zYQapVAbsPyCWM5QKd9GbIcPkzz9Hbw_3r8qlZvTw-LxerxnExL826b5kEtl5bIYH2c-kE7RxwS5zorFMd5YrbHnh9wvCOWmhVy7jlxs6lbS0_RzdT7z7Fz7Hu1juf68GDCRDHrKlkhCvFiKjo9R90G8cU6nWaMTmvFGcHik6USzHnBL3eJ78z6UtTog--9eRbV9_64FurmmFTJlc2bCD9Nv8f-gbpJoON</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Kong, Lisheng</creator><creator>Holtz, Christine T.</creator><creator>Nairn, Campbell J.</creator><creator>Houke, Haley</creator><creator>Powell, William A.</creator><creator>Baier, Kathleen</creator><creator>Merkle, Scott A.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20140401</creationdate><title>Application of airlift bioreactors to accelerate genetic transformation in American chestnut</title><author>Kong, Lisheng ; Holtz, Christine T. ; Nairn, Campbell J. ; Houke, Haley ; Powell, William A. ; Baier, Kathleen ; Merkle, Scott A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-df625e2ddb45e1f95c417ce3b0c47bc871383bfe3016a371be68623b3ab95b6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acceleration</topic><topic>Agrobacterium</topic><topic>Air flow</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Blight</topic><topic>Castanea dentata</topic><topic>Castanea mollissima</topic><topic>Cell culture</topic><topic>Chestnut</topic><topic>Clumps</topic><topic>Cultivation</topic><topic>Dichlorophenoxyacetic acid</topic><topic>Flasks</topic><topic>Genes</topic><topic>Genetic transformation</topic><topic>Genotypes</topic><topic>Glutamine</topic><topic>Hybrids</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Propagation</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Thaumatin</topic><topic>Tissue culture</topic><topic>Tissues</topic><topic>Woody plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Lisheng</creatorcontrib><creatorcontrib>Holtz, Christine T.</creatorcontrib><creatorcontrib>Nairn, Campbell J.</creatorcontrib><creatorcontrib>Houke, Haley</creatorcontrib><creatorcontrib>Powell, William A.</creatorcontrib><creatorcontrib>Baier, Kathleen</creatorcontrib><creatorcontrib>Merkle, Scott A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Lisheng</au><au>Holtz, Christine T.</au><au>Nairn, Campbell J.</au><au>Houke, Haley</au><au>Powell, William A.</au><au>Baier, Kathleen</au><au>Merkle, Scott A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of airlift bioreactors to accelerate genetic transformation in American chestnut</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2014-04-01</date><risdate>2014</risdate><volume>117</volume><issue>1</issue><spage>39</spage><epage>50</epage><pages>39-50</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Airlift bioreactors (ALBs) were constructed and tested for their potential to enhance chestnut embryogenic tissue proliferation for genetic transformation and mass propagation. Multiple genotypes of American chestnut (
Castanea dentata
) and backcross hybrids of American chestnut and Chinese chestnut (
C. dentata
×
Castanea mollissima
) were cultured in ALBs. Medium for tissue proliferation in ALBs was woody plant medium supplemented with 3 g l
−1
sucrose, 0.5 g l
−1
l
-glutamine and 2 mg l
−1
2,4-dichlorophenoxyacetic acid. Optimum culture conditions for 1-l ALBs included 2 % (w/v) tissue inoculation density for culture initiation, 200 ml min
−1
airflow rate, and weekly feeding with fresh medium (80–85 %, v/v). Compared with flasks, ALBs generated higher yields of tissue mass and larger fractions of small cell clumps (<1 mm in diameter) that were optimum target material for transformation. Bioreactor-produced tissue demonstrated high amenability to transformation with reporter genes and chestnut blight resistance candidate genes (CGs) via
Agrobacterium
co-cultivation. The thaumatin-like protein CG was transformed effectively into ALB-produced target material of four genotypes, resulting in thousands of G418-resistant cell clumps, or transgenic events, which reached up to 70 % of the total target cell clumps in liquid selection medium for 3–6 weeks. Substantial acceleration of chestnut transformation was achieved with ALB technology, by which larger quantities of high quality target tissue was produced at lower labor and operating expense than previously used approaches.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-013-0418-8</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2014-04, Vol.117 (1), p.39-50 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| source | Springer Nature |
| subjects | Acceleration Agrobacterium Air flow Biomedical and Life Sciences Bioreactors Blight Castanea dentata Castanea mollissima Cell culture Chestnut Clumps Cultivation Dichlorophenoxyacetic acid Flasks Genes Genetic transformation Genotypes Glutamine Hybrids Inoculation Life Sciences Original Paper Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Propagation Sucrose Sugar Thaumatin Tissue culture Tissues Woody plants |
| title | Application of airlift bioreactors to accelerate genetic transformation in American chestnut |
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