GENES DIFFERENTIALLY EXPRESSED BY Mycobacterium tuberculosis AFTER EXPOSURE TO RUTHENIUM PHOSPHINIC COMPOUND AND ISONIAZID

• Published in: International journal of microbiology research 2013-02, Vol.5 (1), p.357-362
• Main Authors: LEITE, G.G.S., BAEZA, L.C., BATISTA, A.A., BARBOSA, M.I.F., PAVAN, F.R., LEITE, C.Q.F., SILVA, J.L., HIRATA, R.D.C., HIRATA, M.H., CARDOSO, R.F.
• Format: Article
• Language: English
• Subjects: Mycobacterium tuberculosis
• ISSN: 0975-5276; 0975-9174
• DOI: 10.9735/0975-5276.5.1.357-362

The evaluation of the effects of new compounds and nonconventional anti-tuberculous drugs have grown and become increasingly more popular in recent years. Studies have shown anti-tuberculous activity for Ruthenium complexes, including organometallic compounds containing phosphine ligands such as picolinic acid generating great expectations and hopes. The Representational Difference Analysis (RDA) was applied in order to gain insight about differences in expression of Mycobacterium tuberculosis H sub( 37)Rv exposed to (Ru(dppb)(pic)(bypy))PF6 (SCAR1) and isoniazid (INH). RDA-sequencing identified, for both compounds, orthologs that encode hypothetical and predict proteins. One related cell wall synthesis gene, identified by RDA, and genes related to INH target as inhA, katG and ahpC had their expression confirmed and quantified by real-time PCR. The gene encoding the cell wall associated hydrolase was induced 4.627 and 1.189, inhA 0.983 and 1.027, katG 1.111 and 1.345 and ahpC 1.063 and 1.039 fold after exposure to SCAR1 and INH respectively, compared to not exposed growth.