TNF-alpha promoter methylation in peripheral white blood cells: Relationship with circulating TNFα, truncal fat and n-6 PUFA intake in young women

•Women with high truncal fat had lower methylation in some CpG sites of TNFα gene.•The CpG13 methylation level was negatively correlated with plasma TNFα concentrations.•Plasma TNFα concentrations positively correlated with truncal fat.•Daily n-6 PUFA intake was independent predictor of methylation...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 2013-10, Vol.64 (1), p.265-271
Main Authors: Hermsdorff, H.H., Mansego, M.L., Campión, J., Milagro, F.I., Zulet, M.A., Martínez, J.A.
Format: Article
Language:English
Subjects:
5-Methylcytosine - analysis
Adipose Tissue - metabolism
Adiposity - physiology
Adolescent
Adult
Body Composition - physiology
Body Fat Distribution
Body Weight - physiology
Cholesterol, HDL - blood
Cross-Sectional Studies
Diet
Dietary Fats
DNA methylation
DNA Methylation - genetics
Epigenetics
Fatty Acids, Unsaturated - metabolism
Female
Food
Humans
Inflammation
Insulin - blood
Leucocytes
Leukocytes - cytology
Leukocytes - metabolism
Promoter Regions, Genetic - genetics
TNF-alpha
Tumor Necrosis Factor-alpha - blood
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
Young Adult
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Summary:•Women with high truncal fat had lower methylation in some CpG sites of TNFα gene.•The CpG13 methylation level was negatively correlated with plasma TNFα concentrations.•Plasma TNFα concentrations positively correlated with truncal fat.•Daily n-6 PUFA intake was independent predictor of methylation levels in TNFα gene. The aim of this article is to assess the potential relationships between TNFα gene promoter methylation in peripheral white blood cells and central adiposity (truncal fat), metabolic features and dietary fat intake. A group of 40 normal-weight young women (21±3y; BMI 21.0±1.7kg/m2) was included in this cross-sectional study. Anthropometric, biochemical and dietary data were assessed using validated procedures. DNA from white blood cells was isolated and 5-methylcytosine levels of the CpGs sites present in TNFα gene promoter (from −170 to +359 pb) were analyzed by Sequenom EpiTyper. Those women with high truncal fat (⩾52.3%) showed lower 5-methylcytosine levels (P
ISSN:1043-4666
1096-0023
DOI:10.1016/j.cyto.2013.05.028