Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples

The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intr...

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Bibliographic Details
Published in:Research in veterinary science 2012-10, Vol.93 (2), p.682-687
Main Authors: Escribano, D., Gutiérrez, A.M., Martínez Subiela, S., Tecles, F., Cerón, J.J.
Format: Article
Language:English
Subjects:
Accuracy
Animals
Body fluids
IgA, IgG, and IgM
Immunity, Humoral
Immunoassay - methods
Immunoassay - veterinary
Immunoglobulin
Immunoglobulin A - analysis
Immunoglobulin G - analysis
Immunoglobulin M - analysis
Personal relationships
Porcine
Reproducibility of Results
Saliva
Saliva - chemistry
Sensitivity and Specificity
Swine - metabolism
Swine Diseases - diagnosis
Swine Diseases - immunology
Swine Diseases - metabolism
Validation
Veterinary medicine
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Summary:The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals. The clinical validation showed an increase statistically significant (P
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2011.09.018