Search for optimum conditions of wheat straw hemicelluloses cold alkaline extraction process

• Published in: Biochemical engineering journal 2013-02, Vol.71, p.127-133
• Main Authors: García, Juan C., Díaz, M.J., Garcia, M.T., Feria, M.J., Gómez, D.M., López, F.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; cellulose; cold; Cold alkaline extraction; ethanol; Fundamental and applied biological sciences. Psychology; hemicellulose; Hemicelluloses; lignin; raw materials; temperature; Triticum aestivum; Wheat straw; Xylan
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2012.12.008

► A method for the selective extraction of cold alkaline extraction. ► The method for extraction was optimized by using a 2n factor. ► With this method allows 39.4% of all hemicellulose present in the raw material. A method for the selective extraction of hemicellulose from wheat straw involving cold alkaline extraction and subsequent separation by precipitation with ethanol is proposed. Wheat straw affords selective separation of the hemicellulose fraction from the cellulose and lignin fractions with the proposed method. The hemicellulose yield was optimized by using a 2n factor design to examine the influence of temperatures (temperature was designed between 20 and 40°C), operation times (operation time was designed between 30 and 60min) and alkali concentrations (alkali concentration was designed between 80 and 120gL−1). These conditions allowed 56.1% of all hemicellulose initially present in the raw material, and 59.1% of the lignin, to be extracted. Subsequent separation of hemicellulose in the liquid phase from the cold alkaline extraction by precipitation with ethanol provided a fraction containing 39.4% of all hemicellulose (45.2% hemicellulose in extract/total extract) and only 12% of all lignin in the raw material.