Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa

• Published in: Cell calcium (Edinburgh) 2014-04, Vol.55 (4), p.208-218
• Main Authors: Soya, Manabu, Sato, Masaki, Sobhan, Ubaidus, Tsumura, Maki, Ichinohe, Tatsuya, Tazaki, Masakazu, Shibukawa, Yoshiyuki
• Format: Article
• Language: English
• Subjects: Acetanilides - pharmacology; Advanced Basic Science; Animals; Calcium - metabolism; Cell Membrane - physiology; Cells, Cultured; Cricetinae; Hypotonic Solutions - pharmacology; Mechanotransduction; Mechanotransduction, Cellular - drug effects; Merkel cell; Merkel Cells - cytology; Merkel Cells - metabolism; Mouth Mucosa - cytology; Purines - pharmacology; Sensory transduction; Sulfonamides - pharmacology; TRP; TRPC Cation Channels - antagonists & inhibitors; TRPC Cation Channels - metabolism; TRPV Cation Channels - antagonists & inhibitors; TRPV Cation Channels - metabolism
• ISSN: 0143-4160; 1532-1991
• DOI: 10.1016/j.ceca.2014.02.015

Abstract Merkel cells (MCs) have been proposed to form a part of the MC-neurite complex with sensory neurons. Many transient receptor potential (TRP) channels have been identified in mammals; however, the activation properties of these channels in oral mucosal MCs remain to be clarified. We investigated the biophysical and pharmacological properties of TRP vanilloid (TRPV)-1, TRPV2, TRPV4, TRP ankyrin (TRPA)-1, and TRP melastatin (TRPM)-8 channels, which are sensitive to osmotic and mechanical stimuli by measurement of intracellular free Ca2+ concentration ([Ca2+ ]i ) using fura-2. We also analyzed their localization patterns through immunofluorescence. MCs showed immunoreaction for TRPV1, TRPV2, TRPV4, TRPA1, and TRPM8 channels. In the presence of extracellular Ca2+ , the hypotonic test solution evoked Ca2+ influx. The [Ca2+ ]i increases were inhibited by TRPV1, TRPV2, TRPV4, or TRPA1 channel antagonists, but not by the TRPM8 channel antagonist. Application of TRPV1, TRPV2, TRPV4, TRPA1, or TRPM8 channel selective agonists elicited transient increases in [Ca2+ ]i only in the presence of extracellular Ca2+ . The results indicate that membrane stretching in MCs activates TRPV1, TRPV2, TRPV4, and TRPA1 channels, that it may be involved in synaptic transmission to sensory neurons, and that MCs could contribute to the mechanosensory transduction sequence.