Induction of interleukin-2 receptor- alpha gene expression is regulated by post-translational activation of mu B specific DNA binding proteins

T cell mitogens induce the expression of specific trans-acting DNA binding proteins that in turn regulate the expression of the interleukin-2 receptor- alpha (IL-2R alpha ) gene. To investigate whether de novo protein synthesis is required for the activation of these transacting factors and the indu...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-01, Vol.264 (15), p.8475-8478
Main Authors: Boehnlein, E, Ballard, D W, Bogerd, H, Peffer, N J, Lowenthal, J W, Greene, W C
Format: Article
Language:English
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Summary:T cell mitogens induce the expression of specific trans-acting DNA binding proteins that in turn regulate the expression of the interleukin-2 receptor- alpha (IL-2R alpha ) gene. To investigate whether de novo protein synthesis is required for the activation of these transacting factors and the induced expression of this receptor gene, Jurkat T cells were incubated with various inhibitors of protein synthesis prior to stimulation with phytohemagglutinin and phorbol 12-myristate 13-acetate (PMA). The findings support a post-translational mechanism of induction of pre-existing, but inactive, DNA binding proteins which in turn bind to and activate the IL-2R alpha gene.
ISSN:0021-9258