The effect of ileal interposition surgery on enteroendocrine cell numbers in the UC Davis type 2 diabetes mellitus rat

To investigate the short-term effect of ileal interposition (IT) surgery on gut morphology and enteroendocrine cell numbers in the pre-diabetic UC Davis type 2 diabetes mellitus (UCD-T2DM) rat. Two-month old male UCD-T2DM rats underwent either sham (n=5) or IT (n=5) surgery. Intestines were collecte...

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Bibliographic Details
Published in:Regulatory peptides 2014-02, Vol.189, p.31-39
Main Authors: Hansen, Carl Frederik, Vassiliadis, Efstathios, Vrang, Niels, Sangild, Per T., Cummings, Bethany P., Havel, Peter, Jelsing, Jacob
Format: Article
Language:English
Subjects:
Animals
Bariatric surgery
Cholecystokinin - metabolism
Diabetes Mellitus, Type 2 - metabolism
Enteroendocrine cells
Enteroendocrine Cells - cytology
Glucagon-Like Peptide 2 - metabolism
Ileal interposition
Ileum - metabolism
Lipid Metabolism
Male
Rats
Serotonin - metabolism
Stereology
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Summary:To investigate the short-term effect of ileal interposition (IT) surgery on gut morphology and enteroendocrine cell numbers in the pre-diabetic UC Davis type 2 diabetes mellitus (UCD-T2DM) rat. Two-month old male UCD-T2DM rats underwent either sham (n=5) or IT (n=5) surgery. Intestines were collected 1.5months after surgery. The jejunum, ileum and colon regions were processed for histochemical and immunohistochemical labeling and stereological analyses of changes in gut morphometry and number of enteroendocrine cells. Stereological analysis showed that intestinal volume, luminal surface area and the number of all chromogranin A-positive enteroendocrine cells were markedly increased in the IT rats compared with sham-operated animals. Subanalyses of the glucagon-like peptide 2, cholecystokinin, serotonin cells and the neurotensin immunoreactive sub-pool of enteroendocrine cells in the IT region revealed an increase in numbers across phenotypes. However, the density of the different cell types varied. IT surgery in the UCD-T2DM rat leads to rapid alterations in gut morphometry and an increase in the number of enteroendocrine cells. This effect may potentially explain why IT surgery delays the onset of type 2 diabetes in the UCD-T2DM rat. •Ileal transposition leads to marked intestinal adaptation.•Gut hypertrophy and increased enteroendocrine cell numbers following ileal interposition•Intestinal adaptation prevents diabetes onset in a T2D rat model.
ISSN:0167-0115
1873-1686
DOI:10.1016/j.regpep.2014.01.002