Galactose-regulated expression of hepatitis B surface antigen by a recombinant yeast

A yeast expressing the hepatitis B surface antigen (HBsAg) gene under the control of a regulated promoter was grown in batch and fed-batch modes. In batch fermentations, the addition of galactose caused the rapid production of HBsAg. Cells grown in fed-batch mode did not produce HBsAg unless the con...

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Bibliographic Details
Published in:Biotechnology letters 1989-05, Vol.11 (5), p.301-306
Main Authors: Carty, C.E, Tekamp-Olson, P, Rosenberg, S, McAleer, W.J, Maigetter, R.Z
Format: Article
Language:English
Subjects:
antigens
Biological and medical sciences
Biotechnology
Eukaryotes
Fundamental and applied biological sciences. Psychology
galactose
Gene expression
genetic recombination
Genetic technics
hepatitis B surface antigen
Methods. Procedures. Technologies
Molecular and cellular biology
Molecular genetics
Mutant screening
Saccharomyces cerevisiae
viral hepatitis
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Summary:A yeast expressing the hepatitis B surface antigen (HBsAg) gene under the control of a regulated promoter was grown in batch and fed-batch modes. In batch fermentations, the addition of galactose caused the rapid production of HBsAg. Cells grown in fed-batch mode did not produce HBsAg unless the conditioned medium was replaced prior to induction.
ISSN:0141-5492
1573-6776
DOI:10.1007/bf01024508