Antibacterial activity against Clostridium genus and antiradical activity of the essential oils from different origin

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Pesticides, food contaminants, and agricultural wastes, 2014-07, Vol.49 (7), p.505-512
• Main Authors: Kačániová, Miroslava, Vukovič, Nenad, Horská, Elena, šalamon, Ivan, Bobková, Alica, Hleba, Lukáš, Mellen, Martin, Vatľák, Alexander, Petrová, Jana, Bobko, Marek
• Format: Article
• Language: English
• Subjects: Abies alba; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Anti-Bacterial Agents - pharmacology; antibacterial activity; Antimicrobial agents; Antioxidants; Antioxidants - chemistry; antiradical activity; Applied ecology; Bacterial plant pathogens; Biological and medical sciences; Biphenyl Compounds - chemistry; Calendula; Carum carvi; clostridia; Clostridium; Clostridium - drug effects; Clostridium butyricum; Clostridium perfringens; Clostridium ramosum; Diffusion; Disk Diffusion Antimicrobial Tests; Essential oils; Foeniculum vulgare; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control; Gram-positive bacteria; in vitro; Lavandula; Mentha piperita; Oils & fats; Oils, Volatile - pharmacology; Origanum vulgare; Phytopathology. Animal pests. Plant and forest protection; Picrates - chemistry; Pimpinella anisum; Pinus; Pinus sylvestris; Plant Oils - pharmacology; Rosmarinus officinalis; Salvia officinalis; Thymus vulgaris
• ISSN: 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2014.896673

In the present study, the antimicrobial and antiradical activities of 15 essential oils were investigated. The antimicrobial activities were determined by using agar disc diffusion and broth microdilution methods against Clostridium genus and antioxidant properties of essential oils by testing their scavenging effect on DPPH radicals activities. We determined the antibacterial activity of Clostridium butyricum, Clostridium hystoliticum, Clostridium intestinale, Clostridium perfringens and Clostridium ramosum. We obtained the original commercial essential oils samples of Lavandula angustifolia, Carum carvi, Pinus montana, Mentha piperita, Foeniculum vulgare Mill., Pinus sylvestris, Satureia montana, Origanum vulgare L. (2 samples), Pimpinella anisum, Rosmarinus officinalis L., Salvia officinalis L., Abies alba Mill., Chamomilla recutita L. Rausch and Thymus vulgaris L. produced in Slovakia (Calendula a.s., Nova Lubovna, Slovakia). The results of the disk diffusion method showed very high essential oils activity against all tested strains of microorganisms. The best antimicrobial activity against C. butyricum was found at Pimpinella anisum, against C. hystoliticum was found at Pinus sylvestris, against C. intestinale was found at Satureia hortensis L., against C. perfringens was found at Origanum vulgare L. and against C. ramosum was found at Pinus sylvestris. The results of broth microdilution assay showed that none of the essential oils was active against C. hystoliticum. The best antimicrobial activity against C. butyricum was found at Abies alba Mill., against C. intestinale was found at Abies alba Mill., against C. perfringens was found at Satureia montana and against C. ramosum was found at Abius alba and Carum carvi. Antioxidant DPPH radical scavenging activity was determined at several solutions of oil samples (50 μL.mL −1 -0.39 μL.mL −1 ) and the best scavenging effect for the highest concentration (50 μL.mL −1 ) was observed. The antioxidant properties were different in particular plant species. The highest% of inhibition after 30 min. of reaction was observed at Origanum vulgare (93%), Satureia montana (90.66%) and Lavandula augustifolia (90.22%).