Interaction of cupric and cadmium ions with a protein-ovalbumin (OA)

The binding of Cu and Cd ions with ovalbumin has been measured indirectly by observing the displacement of H super(+) from acidic groups on the ovalbumin. The pH-measuring procedure agreed with values obtained by an equilibrium dialysis method. Both the metals were bound by carboxyl groups with log...

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Bibliographic Details
Published in:Water, air, and soil pollution air, and soil pollution, 1989, Vol.43 (1-2), p.53-59
Main Authors: VERMA, S. R, ARORA, J. P. S, SHANKAR, J. S, DEV DUTT, PAL, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Interactions. Associations
Intermolecular phenomena
Molecular biophysics
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Summary:The binding of Cu and Cd ions with ovalbumin has been measured indirectly by observing the displacement of H super(+) from acidic groups on the ovalbumin. The pH-measuring procedure agreed with values obtained by an equilibrium dialysis method. Both the metals were bound by carboxyl groups with log K values being 2.39 and 2.22, respectively. The extent of binding was found to be pH dependent with the involvement of the imidazole site yielding log K values of 3.23 and 2.80, respectively. Equilibrium dialysis results supported involvement of both carboxyl and imidazole groups of the protein in metal ion binding.
ISSN:0049-6979
1573-2932
DOI:10.1007/BF00175582