Immunohistochemical analysis of matrix metalloproteinases (1, 2, and 9), Ki-67, and myofibroblasts in keratocystic odontogenic tumors and pericoronal follicles

• Published in: Journal of oral pathology & medicine 2014-04, Vol.43 (4), p.282-288
• Main Authors: de Oliveira Ramos, Grasieli, Costa, Aline, Meurer, Maria I., Vieira, Daniella S. C., Rivero, Elena R. C.
• Format: Article
• Language: English
• Subjects: Actins - analysis; Basal Cell Nevus Syndrome - metabolism; Basal Cell Nevus Syndrome - pathology; Biological and medical sciences; Cell Proliferation; Connective Tissue - chemistry; Connective Tissue - pathology; Dental Sac - chemistry; Dental Sac - pathology; Dentistry; Epithelium - chemistry; Epithelium - pathology; Humans; Immunohistochemistry; Keratins - analysis; Ki-67 Antigen - analysis; Matrix Metalloproteinase 1 - analysis; Matrix Metalloproteinase 2 - analysis; Matrix Metalloproteinase 9 - analysis; matrix metalloproteinases; Medical sciences; myofibroblasts; Myofibroblasts - pathology; odontogenic tumors; Odontogenic Tumors - chemistry; Odontogenic Tumors - pathology; Otorhinolaryngology. Stomatology; Stromal Cells - chemistry; Stromal Cells - pathology
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/jop.12131

Background Keratocystic odontogenic tumor (KCOT) is a benign tumor that arises sporadically or associated with nevoid basal cell carcinoma syndrome (NBCCS). Its locally aggressive behavior contrasts with its cystic histological appearance. To better understand the interaction between tumor cells and the stroma, the present study aimed to evaluate and compare the immunohistochemical expression of matrix metalloproteinases (MMP‐1, ‐2, and ‐9), the cellular proliferation index (Ki‐67), and the presence of myofibroblasts (MFs) in KCOTs. Methods Eleven cases of isolated KCOT (G1) and 12 cases of KCOT associated with NBCCS (G2) were selected for an immunohistochemical investigation of the proteins MMP‐1, MMP‐2, MMP‐9, Ki‐67, and α‐smooth muscle actin (α‐SMA) in MFs. A group of 6 pericoronal follicles (G3) was included as a normal odontogenic tissue control. Results Significant differences between the G3 and G1/G2 groups regarding the expression of MMP‐1, MMP‐9 (in connective tissue), and Ki‐67 were observed. In KCOT, there was a positive correlation between the Ki‐67 antigen and MMP‐1 and between MFs and MMP‐1 in the parenchyma. No statistical differences were found between G1 and G2 groups. Conclusions MMP‐1, MMP‐9, and proliferative activity appear to play important roles in KCOT pathogenesis. The increased proliferative activity with KCOT was associated with elevated MMP‐1 production in the parenchyma, which influenced the growth of the lesion in association with an increased number of MFs.