Magnetic Porous Sugar-Functionalized PEG Microgels for Efficient Isolation and Removal of Bacteria from Solution

Here, we present a new microparticle system for the selective detection and magnetic removal of bacteria from contaminated solutions. The novelty of this system lies in the combination of a biocompatible scaffold reducing unspecific interactions with high capacity for bacteria binding. We apply high...

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Bibliographic Details
Published in:Biomacromolecules 2013-06, Vol.14 (6), p.1927-1935
Main Authors: Behra, Muriel, Azzouz, Nahid, Schmidt, Stephan, Volodkin, Dmitry V, Mosca, Simone, Chanana, Munish, Seeberger, Peter H, Hartmann, Laura
Format: Article
Language:English
Subjects:
Applied sciences
Bacteria - isolation & purification
Biological and medical sciences
Carbohydrates - chemistry
Composites
Diverse techniques
Escherichia coli
Exact sciences and technology
Forms of application and semi-finished materials
Fundamental and applied biological sciences. Psychology
Gels
Magnetics
Microscopy, Electron, Transmission
Molecular and cellular biology
Nanoparticles
Polyethylene Glycols - metabolism
Polymer industry, paints, wood
Solutions
Technology of polymers
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Summary:Here, we present a new microparticle system for the selective detection and magnetic removal of bacteria from contaminated solutions. The novelty of this system lies in the combination of a biocompatible scaffold reducing unspecific interactions with high capacity for bacteria binding. We apply highly porous poly(ethylene glycol) (PEG) microparticles and functionalize them, introducing both sugar ligands for specific bacteria targeting and cationic moieties for electrostatic loading of superparamagnetic iron oxide nanoparticles. The resulting magnetic, porous, sugar-functionalized (MaPoS) PEG microgels are able to selectively bind and discriminate between different strains of bacteria Escherichia coli. Furthermore, they allow for a highly efficient removal of bacteria from solution as their increased surface area can bind three times more bacteria than nonporous particles. All in all, MaPoS particles represent a novel generation of magnetic beads introducing for the first time a porous, biocompatible and easy to functionalize scaffold and show great potential for various biotechnological applications.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm400301v