Statistical studies on high molecular weight pullulan production in solid state fermentation using jack fruit seed

• Published in: Carbohydrate polymers 2013-10, Vol.98 (1), p.854-860
• Main Authors: Sugumaran, K.R., Sindhu, R.V., Sukanya, S., Aiswarya, N., Ponnusami, V.
• Format: Article
• Language: English
• Subjects: ammonium sulfate; Applied sciences; Artocarpus - growth & development; Artocarpus - metabolism; Aureobasidium pullulans; Biological and medical sciences; Box–Behnken design; carbon; Culture Media - chemistry; Culture Techniques - methods; Exact sciences and technology; experimental design; Fermentation; Fermented food industries; Food industries; Fourier transform infrared spectroscopy; Fundamental and applied biological sciences. Psychology; gel chromatography; Glucans - biosynthesis; Glucans - chemistry; magnesium sulfate; Molecular Weight; Natural polymers; nuclear magnetic resonance spectroscopy; Other fermented food industries; Physicochemistry of polymers; Plackett–Burman design; potassium dihydrogen phosphate; Pullulan; Seeds - metabolism; sodium chloride; solid state fermentation; stable isotopes; Starch and polysaccharides; Statistics as Topic - methods; water content; yeast extract
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.06.071

•Biopolymer pullulan was produced using jack fruit seed.•Solid state fermentation was used.•Medium variables optimized by statistical methods.•34.22mg/g dry substrate of pullulan obtained from jack fruit seed under optimum conditions.•Molecular weight of pullulan from jack fruit seed was 1.73×106g/mol. The purpose of the work was to optimize the medium variables for maximizing pullulan production using jack fruit seed as a low cost substrate by Aureobasidium pullulans in solid state fermentation. Effects of K2HPO4, KH2PO4, ZnSO4·5H2O, MgSO4·7H2O, NaCl, (NH4)2SO4·5H2O, yeast extract, moisture content (%, w/w) in the production medium on pullulan production were studied using Plackett–Burman design. Production of pullulan was significantly affected by the medium variables namely KH2PO4, ZnSO4·5H2O, NaCl and moisture content (%, w/w). Then screened variables were optimized by Box Behnken experiment design. The pullulan obtained was characterized and confirmed by FTIR, 1H NMR and 13C NMR. Molecular weight of pullulan was found to be 1.733×106g/mol by gel permeation chromatography (GPC).