Study of lactoferrin gene expression in human and mouse adipose tissue, human preadipocytes and mouse 3T3-L1 fibroblasts. Association with adipogenic and inflammatory markers

• Published in: The Journal of nutritional biochemistry 2013-07, Vol.24 (7), p.1266-1275
• Main Authors: Moreno-Navarrete, José María, Serrano, Marta, Sabater, Mònica, Ortega, Francisco, Serino, Matteo, Pueyo, Neus, Luche, Elodie, Waget, Aurelie, Rodriguez-Hermosa, José Ignacio, Ricart, Wifredo, Burcelin, Remy, Fernández-Real, José Manuel
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Adipocytes; Adipocytes - metabolism; Adipose tissue; Adipose Tissue - metabolism; Adult; Animals; Biomarkers - metabolism; droplets; enzyme-linked immunosorbent assay; Female; fibroblasts; Fibroblasts - metabolism; gene expression; genes; Humans; Inflammation - metabolism; insulin; Lactoferrin; Lactoferrin - genetics; lipogenesis; Male; messenger RNA; Mice; Middle Aged; noninsulin-dependent diabetes mellitus; Obesity; quantitative polymerase chain reaction; triacylglycerols
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2012.10.002

Lactoferrin is considered an epithelial protein present in different gland secretions. Administration of exogenous lactoferrin is also known to modulate adipogenesis and insulin action in human adipocytes. Here, we aimed to investigate lactoferrin gene expression (real-time polymerase chain reaction) and protein (enzyme-linked immunosorbent assay) levels in human (n=143) and mice adipose tissue samples, in adipose tissue fractions and during human preadipocyte and 3T3-L1 cell line differentiation, evaluating the effects of inducers (rosiglitazone) and disruptors (inflammatory factors) of adipocyte differentiation. Lactoferrin (LTF) gene and protein were detectable at relatively high levels in whole adipose tissue and isolated adipocytes in direct association with low-density lipoprotein-related protein 1 (LRP1, its putative receptor). Obese subjects with type 2 diabetes and increased triglycerides had the lowest levels of LTF gene expression in subcutaneous adipose tissue. Specifically, LTF gene expression was significantly increased in adipocytes, mainly from lean subjects, increasing during differentiation in parallel to adipogenic genes and gene markers of lipid droplets. The induction or disruption of adipogenesis led to concomitant changes (increase and decrease, respectively) of lactoferrin levels during adipocyte differentiation also in parallel to gene markers of adipogenesis and lipid droplet development. The administration of lactoferrin led to autopotentiated increased expression of the LTF gene. The decreased lactoferrin mRNA levels in association with obesity and diabetes were replicated in mice adipose tissue. In conclusion, this is the first observation, to our knowledge, of lactoferrin gene expression in whole adipose tissue and isolated adipocytes, increasing during adipogenesis and suggesting a possible contribution in adipose tissue physiology through LRP1.