Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins

Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)]...

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Bibliographic Details
Published in:Journal of plant physiology 2013-10, Vol.170 (15), p.1303-1308
Main Authors: Aremu, Adeyemi O., Gruz, Jiří, Šubrtová, Michaela, Szüčová, Lucie, Doležal, Karel, Bairu, Michael W., Finnie, Jeffrey F., Van Staden, Johannes
Format: Article
Language:English
Subjects:
acclimation
Acclimatization
antioxidant activity
Antioxidants
Antioxidants - metabolism
benzyladenine
Biochemistry
Collection
Conservation
coumaric acids
Culture
cytokinins
Cytokinins - pharmacology
Derivatives
Hydroxybenzoates - metabolism
isopentenyladenine
Isopentenyladenosine - pharmacology
Liliaceae - drug effects
Liliaceae - metabolism
Medicinal plants
Micropropagation
Phenolic acids
Phytochemicals
plantlets
Plants (organisms)
tissue culture
Topolins
traditional medicine
underground parts
vanillic acid
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Summary:Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)] on the level of phenolic acids and antioxidant activity of M. plumbea during the tissue culture and acclimatization stages. Two cytokinins (mT and mTR) significantly improved the antioxidant activity of tissue culture plantlets while the control plantlets were better after acclimatization. Using UPLC–MS/MS, the levels of hydroxybenzoic and hydroxycinnamic acid derivatives (phenolic acids) varied significantly during tissue culture and acclimatization, depending on the cytokinin and plant part analyzed. Vanillic acid (24.9μgg−1) detected in underground parts of tissue culture plants supplemented with BA was the most abundant phenolic acid detected. The current findings indicate that the phytochemicals together with the bioactivity during in vitro propagation of M. plumbea is influenced by the cytokinin type used and the stage of plant material collection.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2013.04.008