Synthesis and Characterization of a POSS-PEG Macromonomer and POSS-PEG-PLA Hydrogels for Periodontal Applications

• Published in: Biomacromolecules 2014-02, Vol.15 (2), p.666-679
• Main Authors: Wang, David K, Varanasi, Srinivas, Strounina, Ekaterina, Hill, David J. T, Symons, Anne L, Whittaker, Andrew K, Rasoul, Firas
• Format: Article
• Language: English
• Subjects: Animals; Applied sciences; Biological and medical sciences; Cells, Cultured; Exact sciences and technology; Female; Fibroblasts - chemistry; Fibroblasts - cytology; Hydrogels - chemical synthesis; Hydrogels - chemistry; Inorganic and organomineral polymers; Macromolecular Substances - chemical synthesis; Macromolecular Substances - chemistry; Medical sciences; Organosilicon Compounds - chemistry; Particle Size; Physicochemistry of polymers; Polyethylene Glycols - chemistry; Preparation; Rats; Rats, Inbred Lew; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm401728p

A novel water-soluble macromonomer based on octavinyl silsesquioxane has been synthesized and contains vinyl-terminated PEG 400 in each of the eight arms to promote water solubility. The macromonomer was characterized by NMR and FTIR and its aqueous solution properties examined. In water it exhibits an LCST with a cloud point at 23 °C for a 10 wt % aqueous solution. It is surface active with a CMC of 1.5 × 10–5 M in water and in 20:80 v/v acetone/water the CMC is 7.1 × 10–5 M, and TEM images showed spherical 22 nm aggregates in aqueous solution above the CMC. The macromonomer was copolymerized in a 20:80 v/v acetone/water mixture with a vinyl-terminated, triblock copolymer of lactide-PEG-lactide to form a library of cross-linked hydrogels that were designed for use as scaffolds for alveolar bone repair. The cross-linked copolymer networks were shown to contain a range of nm-μm sized pores and their swelling properties in water and PBS at pH 7.4 were examined. At pH 7.4 the hydrogel networks undergo a slow hydrolysis with the release of principally PEG and lactic acid fragments. The hydrogels were shown to be noncytotoxic toward fibroblast cultures at pH 7.4, both initially (days 1–5) and after significant hydrolysis had taken place (days 23–28).