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Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats
This study was designed to investigate the effects of pulsed electromagnetic fields (PEMF) on the balance of adipogenesis and osteogenesis on steroid‐induced osteonecrosis of the femoral head (OFH) in rats. Forty‐two rats were divided into three groups: Steroid group (S, n = 16); Steroid + PEMF grou...
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| Published in: | Bioelectromagnetics 2014-04, Vol.35 (3), p.170-180 |
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| creator | Li, Jian-Ping Chen, Sen Peng, Hao Zhou, Jian-Lin Fang, Hong-Song |
| description | This study was designed to investigate the effects of pulsed electromagnetic fields (PEMF) on the balance of adipogenesis and osteogenesis on steroid‐induced osteonecrosis of the femoral head (OFH) in rats. Forty‐two rats were divided into three groups: Steroid group (S, n = 16); Steroid + PEMF group (S + P, n = 16); and Control group (C, n = 10). For groups S and S + P, all rats were first intravenously given 10 µg/kg lipopolysaccharide on day 1, and then intramuscularly injected with 20 mg/kg methylprednisolone acetate on days 2, 3, and 4, with an interval of 24 h. After 4 weeks, the S + P group was treated with PEMF (4.5‐ms square pulse, repeated at 15 Hz, with a peak of 1.2 mT) for 4 h a day for the next 8 weeks. Group S was not exposed to PEMF. Group C was chosen as the control group, without steroid use and exposure to PEMF. After 8 weeks of treatment, the histological changes, and mRNA and protein expressions of PPAR‐γ2 and Runx2 were measured and analyzed. Compared with the S group, lower incidence of osteonecrosis (31% vs. 69%, P |
| doi_str_mv | 10.1002/bem.21833 |
| format | article |
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Forty‐two rats were divided into three groups: Steroid group (S, n = 16); Steroid + PEMF group (S + P, n = 16); and Control group (C, n = 10). For groups S and S + P, all rats were first intravenously given 10 µg/kg lipopolysaccharide on day 1, and then intramuscularly injected with 20 mg/kg methylprednisolone acetate on days 2, 3, and 4, with an interval of 24 h. After 4 weeks, the S + P group was treated with PEMF (4.5‐ms square pulse, repeated at 15 Hz, with a peak of 1.2 mT) for 4 h a day for the next 8 weeks. Group S was not exposed to PEMF. Group C was chosen as the control group, without steroid use and exposure to PEMF. After 8 weeks of treatment, the histological changes, and mRNA and protein expressions of PPAR‐γ2 and Runx2 were measured and analyzed. Compared with the S group, lower incidence of osteonecrosis (31% vs. 69%, P < 0.05) and empty osteocyte lacuna rate (36.16 ± 15.34 vs. 59.55 ± 21.70, P < 0.01) was observed in the S + P group. Furthermore, PEMF suppressed the expressions of PPAR‐γ2 and improved the expressions of Runx2 in the femoral head (P < 0.05). All data suggest that PEMF is an effective physiotherapy in the treatment of steroid‐induced ONFH, and the possible underlying mechanisms include protecting the balance between adipogenesis and osteogenesis. Bioelectromagnetics. 35:170–180, 2014. © 2014 Wiley Periodicals, Inc.</description><identifier>ISSN: 0197-8462</identifier><identifier>EISSN: 1521-186X</identifier><identifier>DOI: 10.1002/bem.21833</identifier><identifier>PMID: 24421074</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adipogenesis ; Animals ; Core Binding Factor Alpha 1 Subunit - metabolism ; Electromagnetic Fields ; Femur Head - pathology ; Femur Head Necrosis - chemically induced ; Femur Head Necrosis - pathology ; Femur Head Necrosis - physiopathology ; Femur Head Necrosis - therapy ; glucocorticoids ; ischemia necrosis of femoral head ; Kidney - pathology ; Lipopolysaccharides ; Liver - pathology ; Magnetic Field Therapy - instrumentation ; Magnetic Field Therapy - methods ; Male ; Methylprednisolone - analogs & derivatives ; Osteocytes - pathology ; Osteocytes - physiology ; Osteogenesis ; peroxisome proliferator-activated receptor-γ2 (PPAR-γ2) ; PPAR gamma - metabolism ; pulsed electromagnetic fields simulation ; Rats ; Rats, Wistar ; RNA, Messenger - metabolism ; Runt-related transcription factor 2 (Runx2)</subject><ispartof>Bioelectromagnetics, 2014-04, Vol.35 (3), p.170-180</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3393-e6f377f8c14bb9b36304872956e30fbeb26bde83107868e93929be05872f583a3</citedby><cites>FETCH-LOGICAL-c3393-e6f377f8c14bb9b36304872956e30fbeb26bde83107868e93929be05872f583a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24421074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jian-Ping</creatorcontrib><creatorcontrib>Chen, Sen</creatorcontrib><creatorcontrib>Peng, Hao</creatorcontrib><creatorcontrib>Zhou, Jian-Lin</creatorcontrib><creatorcontrib>Fang, Hong-Song</creatorcontrib><title>Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats</title><title>Bioelectromagnetics</title><addtitle>Bioelectromagnetics</addtitle><description>This study was designed to investigate the effects of pulsed electromagnetic fields (PEMF) on the balance of adipogenesis and osteogenesis on steroid‐induced osteonecrosis of the femoral head (OFH) in rats. Forty‐two rats were divided into three groups: Steroid group (S, n = 16); Steroid + PEMF group (S + P, n = 16); and Control group (C, n = 10). For groups S and S + P, all rats were first intravenously given 10 µg/kg lipopolysaccharide on day 1, and then intramuscularly injected with 20 mg/kg methylprednisolone acetate on days 2, 3, and 4, with an interval of 24 h. After 4 weeks, the S + P group was treated with PEMF (4.5‐ms square pulse, repeated at 15 Hz, with a peak of 1.2 mT) for 4 h a day for the next 8 weeks. Group S was not exposed to PEMF. Group C was chosen as the control group, without steroid use and exposure to PEMF. After 8 weeks of treatment, the histological changes, and mRNA and protein expressions of PPAR‐γ2 and Runx2 were measured and analyzed. Compared with the S group, lower incidence of osteonecrosis (31% vs. 69%, P < 0.05) and empty osteocyte lacuna rate (36.16 ± 15.34 vs. 59.55 ± 21.70, P < 0.01) was observed in the S + P group. Furthermore, PEMF suppressed the expressions of PPAR‐γ2 and improved the expressions of Runx2 in the femoral head (P < 0.05). All data suggest that PEMF is an effective physiotherapy in the treatment of steroid‐induced ONFH, and the possible underlying mechanisms include protecting the balance between adipogenesis and osteogenesis. Bioelectromagnetics. 35:170–180, 2014. © 2014 Wiley Periodicals, Inc.</description><subject>Adipogenesis</subject><subject>Animals</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>Electromagnetic Fields</subject><subject>Femur Head - pathology</subject><subject>Femur Head Necrosis - chemically induced</subject><subject>Femur Head Necrosis - pathology</subject><subject>Femur Head Necrosis - physiopathology</subject><subject>Femur Head Necrosis - therapy</subject><subject>glucocorticoids</subject><subject>ischemia necrosis of femoral head</subject><subject>Kidney - pathology</subject><subject>Lipopolysaccharides</subject><subject>Liver - pathology</subject><subject>Magnetic Field Therapy - instrumentation</subject><subject>Magnetic Field Therapy - methods</subject><subject>Male</subject><subject>Methylprednisolone - analogs & derivatives</subject><subject>Osteocytes - pathology</subject><subject>Osteocytes - physiology</subject><subject>Osteogenesis</subject><subject>peroxisome proliferator-activated receptor-γ2 (PPAR-γ2)</subject><subject>PPAR gamma - metabolism</subject><subject>pulsed electromagnetic fields simulation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - metabolism</subject><subject>Runt-related transcription factor 2 (Runx2)</subject><issn>0197-8462</issn><issn>1521-186X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkctu1DAUhi0EokNhwQsgS2xgkdaO7dhZwlAGpHJZAGVn2cnJ1CWxg52o9JV4SjzNdBZISKyO7POd_1x-hJ5SckIJKU8tDCclVYzdQysqSlpQVX2_j1aE1rJQvCqP0KOUrgghShH2EB2VnJeUSL5Cvz_PfYIWQw_NFMNgth4m1-DOQd8mPMYw5QSeLgFb0xvf5AjTNYDHpnVj2IKH5BI2vsUhTXD4CB7nZwyuLZxv5wb2eQ9NDLdAhzsYQjQ9vgTTYrN0GSMUTeh7MybICmYL2HkczZQeowedycM-2cdj9PXt2Zf1u-L80-b9-tV50TBWswKqjknZqYZya2vLKka4kmUtKmCks2DLyragWN5fVQpqVpe1BSIy0wnFDDtGLxbdvPzPGdKkB5cayCN5CHPS-cKcE8Vr_h8o4VQIIVVGn_-FXoU5-rzIjmKiFETRTL1cqN2RUoROj9ENJt5oSvTOa5291rdeZ_bZXnG2A7QH8s7cDJwuwLXr4ebfSvr12Yc7yWKpcNmqX4cKE3_oSjIp9MXHjd7IzZtvUq71BfsD5FHEGA</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Li, Jian-Ping</creator><creator>Chen, Sen</creator><creator>Peng, Hao</creator><creator>Zhou, Jian-Lin</creator><creator>Fang, Hong-Song</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats</title><author>Li, Jian-Ping ; Chen, Sen ; Peng, Hao ; Zhou, Jian-Lin ; Fang, Hong-Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3393-e6f377f8c14bb9b36304872956e30fbeb26bde83107868e93929be05872f583a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adipogenesis</topic><topic>Animals</topic><topic>Core Binding Factor Alpha 1 Subunit - metabolism</topic><topic>Electromagnetic Fields</topic><topic>Femur Head - pathology</topic><topic>Femur Head Necrosis - chemically induced</topic><topic>Femur Head Necrosis - pathology</topic><topic>Femur Head Necrosis - physiopathology</topic><topic>Femur Head Necrosis - therapy</topic><topic>glucocorticoids</topic><topic>ischemia necrosis of femoral head</topic><topic>Kidney - pathology</topic><topic>Lipopolysaccharides</topic><topic>Liver - pathology</topic><topic>Magnetic Field Therapy - instrumentation</topic><topic>Magnetic Field Therapy - methods</topic><topic>Male</topic><topic>Methylprednisolone - analogs & derivatives</topic><topic>Osteocytes - pathology</topic><topic>Osteocytes - physiology</topic><topic>Osteogenesis</topic><topic>peroxisome proliferator-activated receptor-γ2 (PPAR-γ2)</topic><topic>PPAR gamma - metabolism</topic><topic>pulsed electromagnetic fields simulation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - metabolism</topic><topic>Runt-related transcription factor 2 (Runx2)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jian-Ping</creatorcontrib><creatorcontrib>Chen, Sen</creatorcontrib><creatorcontrib>Peng, Hao</creatorcontrib><creatorcontrib>Zhou, Jian-Lin</creatorcontrib><creatorcontrib>Fang, Hong-Song</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioelectromagnetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jian-Ping</au><au>Chen, Sen</au><au>Peng, Hao</au><au>Zhou, Jian-Lin</au><au>Fang, Hong-Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats</atitle><jtitle>Bioelectromagnetics</jtitle><addtitle>Bioelectromagnetics</addtitle><date>2014-04</date><risdate>2014</risdate><volume>35</volume><issue>3</issue><spage>170</spage><epage>180</epage><pages>170-180</pages><issn>0197-8462</issn><eissn>1521-186X</eissn><abstract>This study was designed to investigate the effects of pulsed electromagnetic fields (PEMF) on the balance of adipogenesis and osteogenesis on steroid‐induced osteonecrosis of the femoral head (OFH) in rats. Forty‐two rats were divided into three groups: Steroid group (S, n = 16); Steroid + PEMF group (S + P, n = 16); and Control group (C, n = 10). For groups S and S + P, all rats were first intravenously given 10 µg/kg lipopolysaccharide on day 1, and then intramuscularly injected with 20 mg/kg methylprednisolone acetate on days 2, 3, and 4, with an interval of 24 h. After 4 weeks, the S + P group was treated with PEMF (4.5‐ms square pulse, repeated at 15 Hz, with a peak of 1.2 mT) for 4 h a day for the next 8 weeks. Group S was not exposed to PEMF. Group C was chosen as the control group, without steroid use and exposure to PEMF. After 8 weeks of treatment, the histological changes, and mRNA and protein expressions of PPAR‐γ2 and Runx2 were measured and analyzed. Compared with the S group, lower incidence of osteonecrosis (31% vs. 69%, P < 0.05) and empty osteocyte lacuna rate (36.16 ± 15.34 vs. 59.55 ± 21.70, P < 0.01) was observed in the S + P group. Furthermore, PEMF suppressed the expressions of PPAR‐γ2 and improved the expressions of Runx2 in the femoral head (P < 0.05). All data suggest that PEMF is an effective physiotherapy in the treatment of steroid‐induced ONFH, and the possible underlying mechanisms include protecting the balance between adipogenesis and osteogenesis. Bioelectromagnetics. 35:170–180, 2014. © 2014 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24421074</pmid><doi>10.1002/bem.21833</doi><tpages>11</tpages></addata></record> |
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| subjects | Adipogenesis Animals Core Binding Factor Alpha 1 Subunit - metabolism Electromagnetic Fields Femur Head - pathology Femur Head Necrosis - chemically induced Femur Head Necrosis - pathology Femur Head Necrosis - physiopathology Femur Head Necrosis - therapy glucocorticoids ischemia necrosis of femoral head Kidney - pathology Lipopolysaccharides Liver - pathology Magnetic Field Therapy - instrumentation Magnetic Field Therapy - methods Male Methylprednisolone - analogs & derivatives Osteocytes - pathology Osteocytes - physiology Osteogenesis peroxisome proliferator-activated receptor-γ2 (PPAR-γ2) PPAR gamma - metabolism pulsed electromagnetic fields simulation Rats Rats, Wistar RNA, Messenger - metabolism Runt-related transcription factor 2 (Runx2) |
| title | Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats |
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