Study the biocatalyzing effect and mechanism of cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification

• Published in: Biodegradation (Dordrecht) 2014-06, Vol.25 (3), p.395-404
• Main Authors: Li, Haibo, Guo, Jianbo, Lian, Jing, Xi, Zhenhua, Zhao, Lijun, Liu, Xiaoyu, Zhang, Chenxiao, Yang, Jingliang
• Format: Article
• Language: English
• Subjects: Anthraquinones - chemistry; Aquatic Pollution; Bacteria; Biocatalysis; Biocatalysts; Biodegradation; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cellulose; Cellulose - analogs & derivatives; Cellulose - chemistry; Cellulose acetate; Denitrification; Electrochemical Techniques; Environment and pollution; Fundamental and applied biological sciences. Psychology; Geochemistry; Industrial applications and implications. Economical aspects; Kinetics; Life Sciences; Microbial Consortia - physiology; Microbiology; Nitrites - chemistry; Nitrites - metabolism; Original Article; Oxidation-Reduction; Soil Science & Conservation; Structure-Activity Relationship; Terrestrial Pollution; Waste Management/Waste Technology; Waste Water - chemistry; Waste Water Technology; Water Management; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - metabolism; Water Pollution Control
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-013-9668-8

The biocatalyzing effect of a novel cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification process was studied with anthraquinone, 1,8-dichloroanthraquinone, 1,5-dichloroanthraquinone and 1,4,5,8-tetrachloroanthraquinone. The results showed that the immobilized 1,4,5,8-tetrachloroanthraquinone presented the best biocatalyzed effect which increased nitrite denitrification rate to 2.3-fold with 12 mmol/L 1,4,5,8-tetrachloroanthraquinone. The unequal biocatalyzing effect was due to the quantity and position of –Cl substituent in anthraquinone-structure. Moreover, the nitrite denitrification rate was increased with the oxidation reduction potential (ORP) values becoming more negative during the biocatalyzing process. The stabilized ORP value with 12 mmol/L immobilized 1,4,5,8-tetrachloroanthraquinone were 81 mV lower than the control. At the same time, the more OH − was produced with the higher nitrite removal rate achieved in the nitrite denitrification process. In addition, a positive linear correlation was found between the nitrite removal reaction constants k [gNO 2 − –N/(gVSS d)] and immobilized 1,4,5,8-tetrachloroanthraquinone concentration ( C 1,4,5,8-tetrachloroanthraquinone ), which was k  = 1.8443 C 1,4,5,8-tetrachloroanthraquinone  + 33.75( R 2  = 0.9411). The initial nitrite concentration of 179 mgNO 2 − –N/L resulted in the maximum nitrite removal rate, which was 6.526[gNO 2 − –N/(gVSS d)]. These results show that the application of cellulose acetate immobilized redox mediators (CE-RM) can be valuable for increasing nitrite denitrification rate.