Efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong RNA-silencing suppressor activity of TGBp1

Plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. Here, we report the construction of an expression vector based on plantago asiatica mosaic virus (PlAMV), a member of the genus Potexvirus. Modification of a vector to enhance the express...

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Bibliographic Details
Published in:Archives of virology 2014-05, Vol.159 (5), p.885-896
Main Authors: Minato, Nami, Komatsu, Ken, Okano, Yukari, Maejima, Kensaku, Ozeki, Johji, Senshu, Hiroko, Takahashi, Shuichiro, Yamaji, Yasuyuki, Namba, Shigetou
Format: Article
Language:English
Subjects:
Arabidopsis - virology
Arabidopsis thaliana
Biomedical and Life Sciences
Biomedicine
Cloning
Enzymes
Foot & mouth disease
Foot-and-mouth disease virus
Gene expression
Gene Expression Regulation, Viral - physiology
genes
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Infections
Infectious Diseases
Medical Microbiology
Nicotiana - virology
Nicotiana benthamiana
Original Article
Peptides
Plant Diseases - virology
Plantago
Plantago asiatica mosaic virus
Plasmids
Potato virus X
Potexvirus
Potexvirus - genetics
Potexvirus - metabolism
Proteins
RNA Interference
RNA polymerase
Viral infections
Viral Proteins - metabolism
Virology
Viruses
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Summary:Plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. Here, we report the construction of an expression vector based on plantago asiatica mosaic virus (PlAMV), a member of the genus Potexvirus. Modification of a vector to enhance the expression of a foreign gene, combined with the use of the foot-and-mouth disease virus 2A peptide, allowed efficient expression of the foreign gene in two model plant species, Arabidopsis thaliana and Nicotiana benthamiana. Comparison with the widely used potato virus X (PVX) vector demonstrated that the PlAMV vector retains an inserted foreign gene for a longer period than PVX. Moreover, our results showed that the GFP expression construct PlAMV-GFP exhibits stronger RNA silencing suppression activity than PVX-GFP, which is likely to contribute to the stability of the PlAMV vector.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-013-1860-y