Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro

• Published in: Food chemistry 2013-12, Vol.141 (4), p.3443-3450
• Main Authors: Yogendra Kumar, M.S., Tirpude, R.J., Maheshwari, D.T., Bansal, Anju, Misra, Ksipra
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antimicrobial activity; Antioxidant; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bacteria - drug effects; Bacteria - growth & development; Biological and medical sciences; Hippophae - chemistry; Hippophae rhamnoides; HPLC; Medical sciences; Pharmacology. Drug treatments; Phenol; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves - chemistry; Seabuckthorn
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.06.057

•Preparation of phenolic rich fraction from Seabuckthorn leaves.•Evaluation of antioxidant and antimicrobial activities.•A simple RP-HPLC method for the quantitative analysis of phenolic compounds. Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551–196.89mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r2=0.99), indicating that reducing ability correlated well with amount of PRF. Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.