Lectin-Functionalized Poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) Surface Scaffolds for High Avidity Microbial Capture

Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a...

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Bibliographic Details
Published in:Biomacromolecules 2013-10, Vol.14 (10), p.3742-3748
Main Authors: Hansen, Ryan R, Hinestrosa, Juan Pablo, Shubert, Katherine R, Morrell-Falvey, Jennifer L, Pelletier, Dale A, Messman, Jamie M, Kilbey, S. Michael, Lokitz, Bradley S, Retterer, Scott T
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Biotechnology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Immobilization of organelles and whole cells
Immobilization techniques
Lens culinaris
Lens Plant - chemistry
Methods. Procedures. Technologies
Molecular Structure
Organic polymers
Particle Size
Physicochemistry of polymers
Plant Lectins - chemistry
Plant Lectins - metabolism
Polymethacrylic Acids - chemistry
Polymethacrylic Acids - metabolism
Polyvinyls - chemistry
Polyvinyls - metabolism
Properties and characterization
Pseudomonas fluorescens
Pseudomonas fluorescens - isolation & purification
Special properties (catalyst, reagent or carrier)
Surface Properties
Triticum - chemistry
Triticum vulgare
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Summary:Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a nondestructive method for functional characterization of EPS content. In this report, we evaluate the use of the block copolymer, poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA), as a surface scaffold for lectin-specific microbial capture. Three-dimensional polymer films were patterned on silicon substrates to provide discrete, covalent coupling sites for Triticum vulgare and Lens culinaris lectins. This material increased the number of Pseudomonas fluorescens microbes captured by up to 43% compared to control scaffolds that did not contain the copolymer. These results demonstrate that PGMA-b-PVDMA scaffolds provide a platform for improved microbe capture and screening of EPS content by combining high avidity lectin surfaces with three-dimensional surface topography.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm4011358